摘要:(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu)Typically, 2.5 - 3 volumes of an ethanol/acetate solution is added [阅读全文:]
摘要:1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate (about one week at 21°).2. Macerate two plates of mycelia in a blender with 50 ml of .002 P liquid medium. Inocula[阅读全文:]
摘要:A practical method for the preparation of total DNA from filamentous fungiM.I. Borges, M.O. Azevedo, R. Bonatelli, Jr., M.S.S. Felipe and S. Astolfi-Filho Departamento de Biologia Celular, Universidad[阅读全文:]
摘要:Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 mls0.5 M EDTA 0.24 mls1 M NaCl 3.0 mlsqs to 600 mls dH2OAcetate cushion 3 M NaAcetate pH 4.8 480 μl0.1 % Bromphenol Blu[阅读全文:]
摘要:Tissue collection, storage, microdissection, sectionin g: See separate protocol.Tissue handling : Note that all fresh tissue shoμld be handled as BioSafety Level 2 materials (wear gloves, lab coat,[阅读全文:]
摘要:- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing 10 ml of 1XSE- add 100µg/ml prot[阅读全文:]
摘要:Preparation of Sonicated Salmon Sperm DNA Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps, weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris, pH 7.6. Allo[阅读全文:]
摘要:Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis).Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) [阅读全文:]