摘要:The following guidelines should be taken into account when designing modified oligonucleotides. 1.Sequence Length - SYNTHEGEN can synthesize oligonucleotides from 5 to 110 bases in length. Most sequen[阅读全文]

摘要: Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie, 1979 ([阅读全文]

摘要:1) Digest DNA with two restriction enzymes such that one end is ExoIII susceptible (blunt or 5' overhang) and the other is ExoIII resistant (5' recessed). The ExoIII susceptible end should be such tha[阅读全文]

摘要:Gene cloning or recombinant DNA technology is the joining of two or more segments of DNA to generate a single DNA molecule capable of autonomous replication within a given host (1). Ligase enzymes cat[阅读全文]

摘要:Omar giving a talk at Hunter college; sorry for the poor quaility bc iSight was not behaving as usual." Gene Knock In and Knock Out "[阅读全文]

摘要:Noble lecture of Mario R.Capecchi[阅读全文]

摘要:The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Ideally one would like to work with indi[阅读全文]

摘要:结构基因的结构 人类结构基因4个区域：①编码区，包括外显子与内含子；②前导区，位于编码区上游，相当于RNA5’末端非编码区(非翻译区)；③尾部区，位于RNA3’编码区下游，相当于末端非编码区(非翻译区)；④调控区，包括启动子和增强[阅读全文]

摘要:一般来说，基因克隆技术包括把来自不同生物的基因同有自主复制能力的载体DNA在体外人工连接，构建成新的重组DNA，然后送入受体生物中去表达，从而产生遗传物质和状态的转移和重新组合。因此基因克隆技术又称为分子克隆、基[阅读全文]

摘要:Lysis buffer.The choice of lysis buffer depends on what the cells were labeled with and whether you want to obtain an active kinase.The lowest background is obtained with RIPA buffer without EDTA.EDTA[阅读全文]

摘要:Lysis buffer.The choice of lysis buffer depends on what kind of IP you are doing.RIPA buffer gives the lowest background,but can denature some kinases.It also has the potential to disrupt protein:prot[阅读全文]

摘要:点击下载：General Guide for Cryogenically Storing.PDF[阅读全文]

摘要:Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The basic protocol described below to accomplish this can be divided int[阅读全文]

摘要:1. Introduction. This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene targeted" mice. [阅读全文]

摘要:Introduction PCR protocols to detect rare deletions in DNA from complex populations of mutagenized worms typically depend on a large size differential between the wild-type product and the deletion pr[阅读全文]

摘要:10 ul reactions, amounts per reaction Make MM for 1 extra reaction every 6 *We use parameters that let us see the wild-type product. 30-second extension time generally is adequate for products up to 1[阅读全文]