摘要:Yale Genome Analysis Center, Yale University http://ygac.med.yale.edu/mtn/LS_transf_prot.stm The following protocols were developed by Petra Ross-Macdonald for Mike Snyder's LacZ fusion project at Yal[阅读全文]

摘要:DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Implementation (by Feng Chen, Hau-Qin Pan and Fu Ying) [阅读全文]

摘要:HiI have a 9kb fragment already cloned into bluescript, and I am trying to insert an extra 3.5kb. I've been trying for some time now with no success - when I do get colonies very few have any of the n[阅读全文]

摘要:Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Karin M,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Bio[阅读全文]