摘要:Day 1 Prepare in advance: Chill plates containing bacteriophage-lysed E. coli for at least 1 hour at 4℃ (2 hours works well).Use 2 nitrocellulose filters for primary screening, and 1 filter after that[阅读全文]

摘要:1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Transfer 1.5 mls of culture to eppendorf tube.Spin for 1 minute on high and remove supernatant.3、Add 700 µl STET. Add [阅读全文]

摘要:Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect methylated cytosines using methylation specific PCR (MSP) te[阅读全文]

摘要:Adapted from Frommer et.al.* 1.Dilute DNA (up to 2 mg) into 50 ml with distilled H2O. 2.Add 5.5 ml of 2M NaOH. 3.Incubate at 37℃ for 10 minutes (to create single stranded DNA). 4.Add 30 ml of 10 mM hy[阅读全文]

摘要:Important : Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day. 1.Add 600 m?l of 50 mM [阅读全文]

摘要: Hi everybodyI am having a problem in the lysis of a moderatelly halophilic strain that I am using to prepare DNA. It is Gram negative strain but it resists all kind of things that I tried, any help w[阅读全文]

摘要:BAC提取前准备（前两天 ）配制含25 mg/l Kan的LB培养基选择平板，在超净工作台上用接种环从购买的BAC划线接种至选择平板，于37℃培养箱培养过夜，供选择单。BAC提取前准备（前一天）1、在超净工作台上，用50 µg/ml Kan和[阅读全文]

摘要:I was wondering if anyone could provide an opinion on the difference in PCR amplification effiency from DNA that has been Bisulphite modified using either the MethylEasy Kit or the EZ DNA methylation [阅读全文]

摘要:BIOTECHNOLOGY represents an opportunity for innovative products, new markets, or capital investments, depending on one's perspective.本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:Background Information of RNA iRNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing.[阅读全文]

摘要:1)Place gene of interest between T7 promoters in Òdouble T7Ó plamsid.(slightly more difficμlt alternative:Place gene of interest in hairpin/inverted repeat configuration behind T7 pro[阅读全文]

摘要: Objective:This protocol allows rapid detection of transformation success when primers are available to allow determination of correct ligation products by size or hybridization. Procedures:Start => [阅读全文]

摘要:Materials Expression plasmid (e.g.,pET/Ncd in BL21(DE3)pLysS host cells)ZB = 10 g NZ-Amine A5 g NaCl860 ml final volume10X M9 Salts = 1 g NH4Cl3 g KH2PO46 g Na2HPO4100 ml final volumeM9ZB = 860 ml ZB [阅读全文]

摘要:Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 m[阅读全文]

摘要:How long should cells be labeled?The ideal length of time to label cells depends on the protein of interest and the label that you are using.If you want to label an unstable protein with 35S-methionin[阅读全文]

摘要:Considerations for useThe bicinchoninic acid (BCA)assay is available in kit form from Pierce (Rockford,Ill.).This procedure is very applicable to microtiter plate methods.The BCA is used for the same [阅读全文]

摘要:Considerations for useThe principle of the biuret assay is similar to that of the Lowry,however it involves a single incubation of 20 min.There are very few interfering agents (ammonium salts being on[阅读全文]

摘要:Considerations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minute[阅读全文]

摘要:Bcl-2 might have a membrane transport function by forming ion channels. Conversion of Bcl-2 to a Bax-like death effector by caspases.本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

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