摘要:问：cos-7的转染1、细胞的培养：我们在培养细胞时发现该细胞长的很快，不到2天的时间就长满了；消化的时候老是成块的掉，如果消化时间加长，细胞也能成单个的，但是细胞的生长力又减弱了，请问要怎样来克服这一问题呢？2、转染[阅读全文]

摘要:COS7细胞(猴细胞的一种细胞系)为重组病毒的宿主细胞，是表达SV40大T抗原的非洲绿猴肾细胞株，能支持部分突变型SV40和含SV40 ori的质粒载体的复制,可高水平表达外源基因，常作为瞬时表达宿主。故本实验建立COS7细胞的冻存[阅读全文]

摘要:Caspase家族在介导细胞凋亡的过程中起着非常重要的作用，其中caspase-3为关键的执行分子，它在凋亡信号传导的许多途径中发挥功能。Caspase-3正常以酶原（32KD）的形式存在于胞浆中，在凋亡的早期阶段，它被激活，活化的Caspa[阅读全文]

摘要:Contributor: Suprya JayadevDate: Mar. 11, 1991Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform.2) Place a small portion of glass wool at the base of the column.3[阅读全文]

摘要:问：看了一些关于CSCL梯度离心的资料，但是感觉都不一样，而且过程不清楚。现在想请教一些问题：1、CSCL的密度和转数问题。2、关于CSCL的灭菌。答：因为是要做体内实验，谢谢各位了这是我们实验室的操作步骤：在每4 ml溶解质粒的[阅读全文]

摘要:Assemble the following components: •Cells,grown in 10 cm dishes •Room Temp.PBS •Cold PBS •Cold Sonication Buffer (1% SDS,10 mM EDTA,50 mM Tris-HCl pH 7.5)Prepare sonication buffer [阅读全文]

摘要:文件预览：点击下面的链接下载完整protocol：knockout.doc[阅读全文]

摘要:Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal. 完[阅读全文]

摘要:Before you start, have ready the following: - Sterilize 2 pairs of curved forceps, 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES, with an 18[阅读全文]

摘要:Many methods have been developed to identify genomic targets of DNA-binding factors. Here we outline and discuss our adaptation of the chromatin immunoprecipitation (ChIP) assay to a high-throughput m[阅读全文]

摘要:Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells are performed as described by Boyd and Farnham (MCB 29:8389-8399) and Y. Shang et. al. (Cell 103:843-852) wi[阅读全文]