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搜索“TAG:DNA”找到相关内容217篇,用时1.126014秒

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DNA SEQUENCING (SANGER)
词条创建者:admin创建时间:12-09 23:21
标签: DNA测序

摘要:1. For double-stranded DNA templates:a. Denature template: 10ml DNA (5-10g or alkaline lysis mini prepDNA)8ml ddH2O  2ml 2N NaOH  -incubate 30' at 37℃.b. Dry-ice precipitate: +10ml 4M NH4OAc100ml EtOH[阅读全文]

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DNA重组实验中的常用技术
词条创建者:admin创建时间:12-09 23:21
标签: DNA重组 常用技术

摘要:一、质粒的提取及鉴定(一)质粒的提取及鉴定1、收获细菌(1)将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中,接入一单菌落,于37℃剧烈振荡培养过夜。(2)将1.5ml培养物倒入1.5ml离心管中,用台式离心机于4℃以1[阅读全文]

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DNA Extraction Protocols Using Silica
词条创建者:admin创建时间:12-09 23:20
标签: DNA Extraction Protocols Silica

摘要:Below we present the protocols we have used to isolate DNA from various tissues using Silica and Guanidinium Thiocyanate.These protocols are adapted from Boom et al. (1990),Höss & Pääbo[阅读全文]

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DNA?Precipitation
词条创建者:admin创建时间:12-09 23:20
标签: Precipitation

摘要:Phenol (removes protein) 1.add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) 2.vortex 3.spin 2 minutes at 12000 rpm 4℃4.transfer supernatant to a fresh tube (avoid aspirati[阅读全文]

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DNA from sera-Molecular Biology
词条创建者:admin创建时间:12-09 23:20
标签: sera-Molecular Biology

摘要:I am seeking procedures and/or advice on how to isolate human genomic DNA from platoregistry sera. The sera is lymphocytes that have been frozen for 35-40 years but are not dessicated.Will the isolate[阅读全文]

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DNA extraction from Mutation Detection Enhancement (MDE) Gel
词条创建者:admin创建时间:12-09 23:20
标签: Enhancement

摘要:Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnormal band found by SSCP analysis is normally verified using se[阅读全文]

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DNA Extraction from Frozen Tissue Sections
词条创建者:admin创建时间:12-09 23:20
标签: Extraction

摘要:Tissue collection, storage, microdissection, sectionin g: See separate protocol.Tissue handling : Note that all fresh tissue shoμld be handled as BioSafety Level 2 materials (wear gloves, lab coat,[阅读全文]

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DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
词条创建者:admin创建时间:12-09 23:20
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摘要:- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing 10 ml of 1XSE- add 100µg/ml prot[阅读全文]

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DNA extraction from urine-Molecular Biology
词条创建者:admin创建时间:12-09 23:19
标签: urine-Molecular

摘要:Does anyone have expereince of extracting DNA from urine? I am having problems with this. I have previously extracted the DNA but then it degraded very quickly and since then I havent even been able t[阅读全文]

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DNA fingerprinting - agarose gel【California Institute of Technology】
词条创建者:admin创建时间:12-09 23:19
标签: fingerprinting

摘要:Caltech Genome Research Laboratory,California Institute of Technology http://www.tree.caltech.edu/protocols/agfp.html Restriction digests consist of:15.75 ml ddH2O 1 µl 10 X buffer B (Boehringer[阅读全文]

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DNA precipitation by EtOH &centrifuge-Molecular Biol
词条创建者:admin创建时间:12-09 23:19
标签:

摘要:I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH. I have read about using glycogen as a carrier, I would like to know if anyone knows a method avoiding not to use acrylam[阅读全文]

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DNA extraction from blood-Molecular Biology
词条创建者:admin创建时间:12-09 23:18
标签:

摘要:I am trying to extract DNA from mammalian bloodbut while doing the phenol extractionlot of Gelatinous material in aqeous phase creating problem in its separation.Can somebody tell me how to minimise i[阅读全文]

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DNA重组技术常用酶
词条创建者:admin创建时间:12-09 23:18
标签: DNA重组 常用酶

摘要:限制性核酸内切酶 :识别DNA特定序列,切断DNA链 ;DNA聚合酶Ⅰ: 或Klenow1、缺口平移制作标记DNA探针; 2、合成cDNA的第二链;3、填补双链DNA3’凹端;4、DNA序列分析耐热DNA聚合酶(Taq DNA聚合酶等) 聚合酶链反应(PCR);D[阅读全文]

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DNA的限制性内切酶酶切分析
词条创建者:admin创建时间:12-09 23:18
标签: 内切酶 酶切

摘要:限制性内切酶能特异地结合于一段被称为限制性酶识别序列的DNA序列之内或其附近的特异位点上,并切割双链DNA。它可分为三类:Ⅰ类和Ⅲ类酶在同一蛋白质分子中兼有切割和修饰(甲基化)作用且依赖于ATP的存在。Ⅰ类酶结合于识别[阅读全文]

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DNA重组实验常见问题分析
词条创建者:admin创建时间:12-09 23:18
标签: DNA重组

摘要:TaKaRa的内切酶和NEB的内切酶哪个更好一些? 参考见解: TaKaRa的内切酶、NEB的内切酶两个公司的酶的品质都非常好。NEB公司的酶的活性很高,切出两条小带可能是因为出现星活性,可以试试把酶量减半。NEB的酶很多都是克隆的,[阅读全文]

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DNA Solutions (DNA分离)
词条创建者:admin创建时间:12-09 23:18
标签: DNA分离

摘要:Table of Contents10X TBE 40% Acrylamide Stock Alkaline lysis solution 10X TBE:216 g Tris base 110 g boric acid 16.6 g EDTA Add water to 2 liters.40% Acrylamide/Bisacrylamide (40% A&B):38 g Acrylamide [阅读全文]

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DNA extraction from blister rust aeciospores and urediniospores using dry grindi
词条创建者:admin创建时间:12-09 23:18
标签:

摘要:Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Molecular Pathologist,U.S.D.A. Forest Service, North Central Forest Experiment Station,Forestry Sciences Laboratory, 5985 Hw[阅读全文]

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DNA?Extraction?from?Archival?Formali
词条创建者:admin创建时间:12-09 23:17
标签:

摘要:DNA Extraction from Archival Formalin-fixed, Paraffin-embed allowfullscreen='true'ded Tissue SectionsAuthor: Shi et al. Source: Contributed by APostodoc Abstract: Describes two methods of extracting D[阅读全文]

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DNA分子克隆
词条创建者:admin创建时间:12-09 23:17
标签: DNA分子克隆

摘要:在体外将DNA分子片段与载体DNA片段连接,转入细胞获得大量拷贝的过程中DNA分子克隆(或基因克隆)。其基本步骤包括:制备目的基因→将目的基因与载体用限制性内切酶切割和连接,制成DNA重组→导入宿主细胞→筛[阅读全文]

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DNA isolation from Avian tissue-Molecular Biology
词条创建者:admin创建时间:12-09 23:17
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摘要: I am working with birds of prey....i am looking for a simple method for isolating genomic DNA from avian breast muscle....I have found protocols for mammalian tissue...will these work fine? also i ha[阅读全文]

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