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搜索“TAG:DNA”找到相关内容217篇,用时1.144543秒

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DNA序列分析(DNA sequencing)
词条创建者:admin创建时间:12-09 23:14
标签: DNA序列

摘要:DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA.The most popular method for doing this is called the dideoxy method.The equipment and supplies:·Ste[阅读全文]

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DNA?Purification?from?Gels
词条创建者:admin创建时间:12-09 23:14
标签: Purification

摘要:1.Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc,pH 7.0 300 ml 1 M NaOAC,pH 7.01 mM EDTA 2 ml 500 mM EDTA,pH 8.0698 ml ddH202.Incubate at 22℃ for 30 min.Transfer gel sli[阅读全文]

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DNA-Methyltransferase?Assay
词条创建者:admin创建时间:12-09 23:14
标签: DNA-Methyltransferase

摘要:This protocol was written by Jean-Pierre Issa,based on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity [阅读全文]

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DNA甲基化研究方法的回顾与评价(图)
词条创建者:admin创建时间:12-09 23:14
标签: DNA甲基化

摘要:摘要: DNA 甲基化是表观遗传学(Epigenetics)的重要组成部分,在维持正常细胞功能、遗传印记、胚胎发育以及人类肿瘤发生中起着重要作用,是目前新的研究热点之一。随着对甲基化研究的不断深入,各种各样甲基化检测方法被开[阅读全文]

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DNA Isolation from Agarose Gels with DEAE Paper
词条创建者:admin创建时间:12-09 23:13
标签: Agarose

摘要: MATERIALS:The DEAE paper can be used as supplied by Schleicher and Schuell, however the binding capacity can be increased by washing the paper in 10mM EDTA, pH 7.5 for 10 minutes, 0.5M NaOH for 5 min[阅读全文]

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DNA Preparation from Adherent Cells
词条创建者:admin创建时间:12-09 23:13
标签: Preparation Adherent

摘要:Section of Cancer Genomics,Genetics Branch,NCINational Institutes of HealthReagentsChloroformMallinckrodt,cat.4440EDTA,0.5 MEthanol,100%Ethanol,70%Isoamyl AlcoholSigma,cat.I-3643PhenolGibco BRL,Cat.15[阅读全文]

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DNA?methylation
词条创建者:admin创建时间:12-09 23:13
标签: methylation

摘要:DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group,catalyzed by DNA methyltransferase (DNMT),to the 5-c[阅读全文]

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DNA Ligations
词条创建者:admin创建时间:12-09 23:13
标签:

摘要:Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation http://omrf.ouhsc.edu/~frank/LIGATION.html [阅读全文]

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DNA?Purification?and?Precipitation
词条创建者:admin创建时间:12-09 23:13
标签: Purification Precipitation

摘要:1.Prepare or obtain Buffered phenol,pH 8.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored.2.Combine DNA sample with an equal volume of[阅读全文]

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DNA的琼脂糖纯化(John Roth, Division of Biological Sciences,U
词条创建者:admin创建时间:12-09 23:13
标签: 琼脂糖 糖纯化

摘要:1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Locate bands with a hand-held long-wave UV lamp.2.Slice the gel with a razor blade above and below the bands of interest[阅读全文]

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DNA?Fragment?Purification
词条创建者:admin创建时间:12-09 23:13
标签: Fragment Purification

摘要:Materials:1.TE solutiono10 mM Tris (pH to 7.5)o1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose gel piece containing the desired DNA fragmentSupplies:1.Micropipetter and tips2.Microcentrifuge and tub[阅读全文]

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DNA?and?RNA?EXTRACTIONS
词条创建者:admin创建时间:12-09 23:13
标签:

摘要:A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also1)Take one medium sized leaf or half a large leaf [阅读全文]

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DNA?From?Whole?Blood?for?PCR
词条创建者:admin创建时间:12-09 23:13
标签: Blood PCR

摘要:1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube.Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA.Mix immediatel[阅读全文]

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DNA连接技术
词条创建者:admin创建时间:12-09 23:12
标签: DNA连接

摘要:[原理]利用DNA 连接酶把载体 DNA 和要克隆的目的 DNA 片段连接在一起,成为一个完整的重组分子,这就是分子克隆中常说的连接反应。当载体 DNA 和外源 DNA 末端都是由一种产生粘性末端的内切酶切割产生的话(同尾酶也可以[阅读全文]

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DNA回收问题解答
词条创建者:admin创建时间:12-09 23:12
标签: 常见问题及对策

摘要:1.没有回收到 DNA 片段如在洗脱后,发现洗脱液中没有 DNA 片段,请检查是否按瓶身标签,在洗涤液中加入了无水乙醇.2.提取率低(1)融胶液为酸性缓冲液,如融胶后,其pH 值升高将影响提取得率.请加入0.1 倍体积的3M乙酸钾(pH[阅读全文]

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DNA指纹图谱分析[DNA Fingerprinting ](图)
词条创建者:admin创建时间:12-09 23:12
标签:

摘要:一.实验目的 1.掌握DNA指纹图谱技术的概念、原理和基本操作过程2.学习DNA的限制性酶切的基本技术3.掌握琼脂糖凝胶电泳的基本操作技术,学习利用琼脂糖凝胶电泳测定DNA片段的长度,并能对实验结果进行分析。二.实验原理19[阅读全文]

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DNA的纯化浓缩与定量
词条创建者:admin创建时间:12-09 23:12
标签: DNA的纯化

摘要:实验目的 将复杂的细胞分子混合物加入有机溶媒萃取以除去蛋白质及其它成分,就可以纯化DNA。一般常用酚及氯仿(phenol/chloroform)可使蛋白质变性(denaturaion)的特性来进行萃取的步骤,DNA和RNA不溶于有机溶媒中,而溶于[阅读全文]

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DNA Isolation from Tails
词条创建者:admin创建时间:12-09 23:12
标签: DNA提取

摘要:Jacks Lab,Center for cancer research,MIT 1.Each tail should be in a clean eppendorf tube.2.Add 500µl of tail lysis buffer containing Proteinase K (PK)to each tube.3.Incubate tail samples in 5060[阅读全文]

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DNA?Fragment?Purification?from?Agaro
词条创建者:admin创建时间:12-09 23:12
标签:

摘要:DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal.For smaller fragments (20 bp to 400 bp)the acrylamide purification is preferred[阅读全文]

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DNA限制性内切酶消化
词条创建者:admin创建时间:12-09 23:12
标签: 限制性内切酶

摘要:[原理]DNA 限制性内切酶消化是基因分析中的关键步骤。内切酶是最关键的工具酶。限制性内切酶是一类具有严格识别位点,并在识别位点内或附近切割双链DNA 的脱氧核糖核酸酶。酶单位规定为:在最适反应条件下 1 小时完全消化[阅读全文]

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