摘要:Mirmira Laboratory at the University of VirginiaThere are multiple variations to this protocol, but we find that this one works well in all cases we tested.http://faculty.virginia.edu/mirmira/resource[阅读全文]

摘要:SolutionsProtocol: Digest 5-10 μg genomic DNA overnight with restriction enzyme of choice.Run digested gDNA on 0.8% TAE gel with marker (with no ethidium bromide).Transfer Setup: 1. Remove gel and [阅读全文]

摘要:I am trying to digest mouse genomic DNA for a southern blot. When digested with either HIndIII or NdeI, I get a nice smear as expected. But when I digest with either speI or BglII (neither one of whic[阅读全文]

摘要:1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at rosette stage if possible). Use 96 well plates (1 or 2ml, E&K, pol[阅读全文]

摘要:What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we are separating molecules of DNA that we got from [阅读全文]

摘要:Omar giving a talk at Hunter college; sorry for the poor quaility bc iSight was not behaving as usual." Gene Knock In and Knock Out "[阅读全文]

摘要:Noble lecture of Mario R.Capecchi[阅读全文]

摘要:The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Ideally one would like to work with indi[阅读全文]

摘要:Description: For each SNP two PCR primers and a forward and reverse single base extention SNP primer design was attempted. After obtainign uniquely mapped SNPs with other known SNPs marked in the sequ[阅读全文]

摘要:SolutionsElution Buffer50 mM Tris 7.5 5 ml 1M Tris pH 7.50.1% SDS 0.1 g SDS0.1 mg/ml BSA 1.0 mg BSA0.25 mM EDTA 50 ml 0.5M EDTA2.5% glycerol 2.5 ml glycerolup to 100 ml with QFor every 10 ml add:10 ml[阅读全文]

摘要:1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃2)extract cells (from 500ml)in 25 mls.Heintz Buf[阅读全文]

摘要:结构基因的结构 人类结构基因4个区域：①编码区，包括外显子与内含子；②前导区，位于编码区上游，相当于RNA5’末端非编码区(非翻译区)；③尾部区，位于RNA3’编码区下游，相当于末端非编码区(非翻译区)；④调控区，包括启动子和增强[阅读全文]

摘要:一般来说，基因克隆技术包括把来自不同生物的基因同有自主复制能力的载体DNA在体外人工连接，构建成新的重组DNA，然后送入受体生物中去表达，从而产生遗传物质和状态的转移和重新组合。因此基因克隆技术又称为分子克隆、基[阅读全文]

摘要:为了支持公共使用和散布基因表达数据，NCBI开始了基因表达汇编（GEO）计划。GEO是努力建立一个基因表达数据仓库和在线资源，用于从任何物种或人造的来源检索基因表达数据。来自microarray，高密度寡核苷酸array（HAD），杂交膜[阅读全文]

摘要:GenBank包含所有已知的核苷酸及蛋白质序列、以及与之相关的生物学信息和参 考文献，是美国生物技术信息中心（NCBI）建立并维护的，是世界上的权威序列数据 库。数据库序列的来源为作者直接递交或间接查寻文献所得，并与世界[阅读全文]

摘要:Genscan程序是通过设计基因序列模型来得到真核生物的基因。其编码区使用五阶的马可夫模型，而不使用来自同源信息的模型。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:Genscan程序是通过设计基因序列模型来得到真核生物的基因。其编码区使用五阶的马可夫模型，而不使用来自同源信息的模型，使得Genscan的结果不依靠于目前的蛋白库中的相似基因，从而提供了于同源基因识别不一样的方法。本[阅读全文]

摘要:Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS Extraction Buffer1 M Tris-HCl pH 7.510.00 ml5 M NaCl2.50 ml0.5 M EDTA2.50 ml20% SDS1.25 mlH2O33.75 mlTotal50.00 [阅读全文]

摘要:This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are made that any of the steps are necessary or ideal; th[阅读全文]

摘要:1.目的：建立一个GZX-GF-OSM型电热恒温鼓风干燥箱使用、维护保养与清洁标准操作程序，使操作过程标准化2.职责：质量部QC负责本文件的起草，质量部及QC检验人员负责本标准的实施。3.范围：本标准适用于GZX-GF-OSM型电热恒温[阅读全文]