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搜索“TAG:G”找到相关内容78篇,用时0.015574秒
摘要:心得如下:1、 GES长的非常快,而且比较大,呈成纤维细胞样,细胞长得越好,铺的越开。2、传代一般1:3传,每3天需要再传。2、永生化细胞,不要等细胞100%汇合再传代,多次这样细胞容易球形增加,还不容易洗掉。3、用胰酶(0.25%)--EDT[阅读全文]
摘要:zhaolifei认为:分析转化的功能和表达需要DNA稳定转染至宿主细胞染色体。外源基因进入细胞后,部分能够通过细胞质进入细胞核内,根据细胞类型,至多80%的进入核内的外源DNA得到瞬时表达。极少数情况下,进入细胞的外源DNA通[阅读全文]
摘要:ctyw708求助GenBank使用的疑问众所周知,引物设计常常用到GenBank,但是我查到的是mRNA的序列,用的软件Primer Premier 5.0,里面要输入的是DNA sequence,该如何转换,按照碱基配对原则进行变换(reverse complemently)就[阅读全文]
摘要:tjmedwnn:关于G418筛选的问题大家好,请问我用G418筛选转染细胞,首先做最佳浓度确定实验,为什么要取5天杀死大量细胞,14天杀死全部细胞的最小量,我的问题是,为什么取5天,而不是3天或4天2天的,WHY?做最佳浓度确定实验加[阅读全文]
摘要:问:我最近对肿瘤细胞转染GFP,其中不含有其他外源性基因。转染后短期内发现阳性细胞非常多,可是随着时间及细胞的增殖,培养孔内的细胞总数在不断的增加,但是发绿色荧光的细胞反而较以前少了(培养液中一直加着G418)。另外[阅读全文]
摘要:问:最近要做稳定转染了,但不知道如何配制G418,有一些细节想问问大家,我打算用PBS来配,我想问问是用高压过的PBS来配,还是用未高压的PBS配,配完了是不是要过滤,然后放到-20保存,用的时候是不是放在4度.请各位来帮我解[阅读全文]
摘要:The early male and female embryos are very similar and differentially develop in latter stages. 本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:1.G418的配制:取1g G418溶于1ml 1M的HEPES液中,加蒸馏水至10ml,过滤消毒,4度保存。2.细胞培养:取待测培养细胞,制备成细胞悬液,按等量接种入多孔培养板中,培养6小时左右开始加药。3.制备筛选培养基:在100ug/ml~1000ug/m[阅读全文]
摘要:Contributor: Suprya JayadevDate: Nov. 10, 19931) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containi[阅读全文]
摘要:1. Introduction. This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene targeted" mice. [阅读全文]
摘要:Introduction PCR protocols to detect rare deletions in DNA from complex populations of mutagenized worms typically depend on a large size differential between the wild-type product and the deletion pr[阅读全文]
摘要:10 ul reactions, amounts per reaction Make MM for 1 extra reaction every 6 *We use parameters that let us see the wild-type product. 30-second extension time generally is adequate for products up to 1[阅读全文]
摘要:完整的实验方法请点击下面的链接下载:09G-banding for analysing mouse chromosomal rearrangements.pdf[阅读全文]
摘要:This is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with DNA genotyping and who need to establish a genotyping method.[阅读全文]
摘要:This is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with DNA genotyping and who need to establish a genotyping method.[阅读全文]
摘要:试剂Earles Balanced Salt Solution (BSS) earlesFetal Bovine SerumGiemsa stain, original azure blend GiemsaHarleco 620G/75 harleco 620g/75Gurrs buffer tablets, pH 6.8 gurrsBiomedical Specialties, Santa [阅读全文]