摘要:在非临床有效性研究过程中，主要采用行为学试验研究药物对动物学习记忆功能的影响。人和动物的内部心理过程是无法直接观察到的，只能根据可观察到的刺激反应来推测脑内发生的过程，对脑内记忆过程的研究只能从人类或动物[阅读全文]

摘要:一、系统简介：TruScan系统可以精确的监测动物在笼子内的活动状态，配合电击板、探鼻板、明暗盒等使用，适用于研究药物或外界刺激对动物记忆、情绪（如恐惧、抑郁等）和行为等的影响。该系统能保留原始资料，可用不同方案进行多[阅读全文]

摘要:一、概述T7 DNA聚合酶最初具有5'→3'聚合酶活性以及单链和双链3'→5'外切酶活性。当T7 DNA聚合酶用适当方法处理后，可使3'→5'外切酶活力明显下降。改造后的T7 DNA聚合酶又称T7测序酶。使用T7测序酶得到的测[阅读全文]

摘要:The quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG precipitation procedure (the Core can supplyinformation on this protocol). [阅读全文]

摘要:TOSOH公司是世界上专门从事生产生物样品分离介质的公司之一，其最著名的产品是TSK-GEL色谱柱及其填料，已经在世界范围内非常多的实验室和工厂使用。大量的数据表明该产品非常适合生物及相关样品的分析分离。TSK-GEL SW系[阅读全文]

摘要:Tandem mass spectrometry (MS/MS) now plays a very important role in protein identification due to its fastness and its high sensitivity.The derivation of the peptide sequence from its MS/MS spectrum i[阅读全文]

摘要:T7噬菌体晚期转录系统是特殊的表达系统，其启动子为III型启动子，不能被宿主大肠杆菌RNA聚合酶识别，只为噬菌体本身编码的T7 RNA聚合酶所识别。近年又发现了T7 型启动子也能被哺乳类动物细胞转录系统所启动。原核生物和真[阅读全文]

摘要:Table of Contents 1、 Introduction - What’s an ELISA?2、 Assay Formats - What are my choices?3、 How to Choose an Assay Format - I know what I want to measure. What do I do?4、 Factors to Consider W[阅读全文]

摘要:Rare event analysis in flow cytometry is nothing new. Why the statistics involved have been overlooked.本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:T细胞亚群的分离分离T细胞亚群，包括各种CD抗原阳性的亚群、αβ型T细胞受体阳性的T细胞或γδ型T细胞、或者其它表面标志不同的T细胞亚群，通常需要有相应的特异性抗体。用直接或间接免疫荧光染色，通[阅读全文]

摘要:MaterialsMonolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used, or cultures of tetrahymena, yeast, or algae may be used.)H-thymidine with at least 4 µc/ml, 0.36 [阅读全文]

摘要:We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result, most of our incu[阅读全文]

摘要:Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the back[阅读全文]

摘要:MS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0gMOPS..............0.5gAgar..............8.0gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3.[阅读全文]

摘要:METHOD:1.Established hybridomas and other cell lines can be stored in liquid N2 using this method.2.Have everything ready before you start working with the cells (i.e. cool media, label vials, prepare[阅读全文]

摘要:Seed cells at appropriate density (5x104/well for 3T3, MPAC, BHK; 5x105/well for aTC1.6, bTC3) one to two days before transfection. Combine total DNA at 1ug /well with 600ul/well serum-free medium in [阅读全文]

摘要:THE PURINE NUCLEOTIDE CONTENT IN HUMAN LEUKEMIA CELL LINESAbstract: The HPLC method was used to determine the purine nucleotide (ATP, ADP, AMP, GTP, GDP, GMP, NAD+) contents and the values of the aden[阅读全文]

摘要:INTRODUCTIONMeasurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts i[阅读全文]

摘要:ECACC and Human Genetic ResearchThe European Collection of Cell Cultures (ECACC) was first established within CAMR, Salisbury, UK in 1984 and has grown to be one of the largest animal cell culture col[阅读全文]

摘要:In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. Over the last t[阅读全文]