摘要:pSico Oligo Design Click on the Mac OSX program pSicoOligomaker 1.5 to select and design oligos for pSico and plentilox3.7.To operate it, simply paste your sequence in the "sequence" window, give it a[阅读全文]

摘要:RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercaptoethanol1. Grind leaf tissue on liquid N in mortar and pestle.2. Transfer the ground tissue to a 10ml conical botto[阅读全文]

摘要:Overview We design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse Refseq transcript. The algorithm applies a set of [阅读全文]

摘要: T-RFLP--末端限制性片段长度多样性，又称为16sRNA基因的末端限制性片段分析技术。是一种新兴的研究微生物多态性的分子生物学方法，日亦受到研究人员的重视。该技术已经成功应用于各种微生物群落的分析比较、研究微生物群[阅读全文]

摘要:T-RFLP（末端限制性片段长度多态性）该技术在应用的过程中，肯定需要在实验条件上不断进行改进，而这种改进的好坏自然而然需要实验结果的验证。V. Grüntzig在2002年做了该工作的一部分，结果认为，在限制性酶切时，很有[阅读全文]

摘要:General Advice PCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this technique means that the sample should not be contam[阅读全文]

摘要:Topics　　• What is the Polymerase Chain Reaction?　　• History and (pre-history) of PCR　　• How PCR works　　• Optimising PCR　　• Fidelity, errors and cloning　　• PCR primer desi[阅读全文]

摘要:Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.* The 10x PCR buffer contains: 500 mM KCl; 100 mM Tris-HCl (pH 8.3); 15 mM [阅读全文]

摘要:SYBR Green Ⅰ荧光染料：是一种只与双链DNA小沟结合的染料，当它与DNA双链结合时，发出荧光；从DNA 双链上释放出来时，荧光信号急剧减弱。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。在PCR 反应体系中，加[阅读全文]

摘要:点击下面链接下载TaqMan探针法SNP筛查中文操作手册.pdf [阅读全文]

摘要:Western- or immunoblotting is a commonly employed technique for the detection of protein antigens in complex mixtures. Samples are first separated by SDS-polyacrylamide gel electrophoresis. The separa[阅读全文]

摘要:Storage of lyophilized antibodies All our products are shipped lyophilized (freeze-dried). Unlabeled antibodies are stable without loss of quality at ambient temperatures for several weeks or even a f[阅读全文]

摘要:The following tips can be used to overcome the most common problems encountered during Western blotting. Smeared Pattern or Distorted Bands Uneven contact between gel and membrane: cassettes used shou[阅读全文]

摘要:To concentrate proteins for analysis by SDS PAGE:If a small amount of protein is to be precipitated (less than a few micrograms),add Insulin as a carrier protein (10 micrograms of Sigma insulin,I-5500[阅读全文]

摘要:Myeloid DC1s are located at sites of pathogen entry such as skin and mucosal tissues,and may recognize and present a much broader range of antigens or pathogens.本篇文章来源于网络，如有异议请联系我们，[阅读全文]

摘要:Thp接触抗原分化成一种不确定的状态，若Thp接触抗原及多种信号综合作用下，使Thp分化成Th1和Th2。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:跑凝胶电泳，然后将蛋白质从凝胶转移到硝酸纤维素膜中再用考马斯蓝将凝胶染色，对比几组结果。[阅读全文]

摘要:TOM：在线粒体外膜上的转位酶复合体，使外界蛋白质转位进入线粒体。[阅读全文]

摘要:一、操作程序1、使用前必须先检查面板上的各旋钮是否在规定的位置上（即电源在关的位置上，电位器及定时器在零的位置上）。2、每支试管中放置等量的样品，然后对称放入转头内，以免由于重量不均，放置不对称，而使整机在运行过[阅读全文]

摘要:TCP2系列色差仪使用方法色差计使用之前，应先检查电源电缆、传感器接口是否连接正确，以及检查功能选择开关是否设定为便携式测量方式。检查完毕后，方可打开仪器开关进行测量操作。具体操作如下：1、将传感器电缆插入仪器背[阅读全文]