按字母顺序浏览:A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 其他
搜索“TAG:T”找到相关内容100篇,用时0.020505秒
摘要:TE(PH8.0): 10 mmol/LTris-Cl,1mmol/L EDTA(pH8.0) DNA磷酸骨架露在外面,因此核酸的外侧有负电聚集;若把 DNA 溶在纯水里,则两股磷酸脊骨会互相排斥,造成 DNA 的两股分开而变性沉淀,故 DNA 都要溶在含有离子的溶液中 [阅读全文]
摘要:【求助】关于TGF-Beta,FN酶联免疫(ELISA)试剂盒的选择参与者:txl4fox我是要酶联免疫(ELISA)的方法测细胞上清液中的TGF-Beta,FN抗原的表达情况,关于ELISA试剂盒的选择我想请教几个问题:我想买酶标板自己包被一抗,那我应[阅读全文]
摘要:1.材料和试剂1.1培养液A :DMEM培养液添加10%的胎牛血清(FBS)。1.2培养液B:DMEM培养液添加10%的胎牛血清(FBS)和终浓度为0.7--1μg/ml的放线菌素D。1.3L929细胞株:鼠结缔组织纤维母细胞,来源于22天雄性C3H鼠皮下组织。1[阅读全文]
摘要:Tris饱和苯酚(Tris saturated phenol)也称Tris平衡酚(Tris balanced phenol),是添加了抗氧化剂8-羟基喹啉,tris pH8.0充分平衡的苯酚,呈黄色。常用于DNA抽提和纯化等。重蒸酚是苯酚在182度蒸馏的组分。tris配制方法:1、取[阅读全文]
摘要:第一节 概念这里想明确两个概念,一个是Transwell,另一个是肿瘤细胞侵袭模型。1.Transwell关于Transwell这个词该如何解释,查了很多资料也未见准确的注解,我觉得可以这么理解吧,trans-这个词根有转移、转运、穿过等意思,w[阅读全文]
摘要:一、原理与意义:TUNEL(TdT-mediated dUTP nick end labeling)细胞凋亡检测试剂盒是用来检测组织细胞在凋亡早期过程中细胞核DNA的断裂情况,其原理是生物素biotinylate标记的dUTP在脱氧核糖核苷酸末端转移酶(TdT Enzyme[阅读全文]
摘要:信号传导(cell signalling) 是细胞通讯的基本概念,强调信号的产生、分泌与传送,即信号分子从合成的细胞中释放出来,然后进行传递。本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:脓毒症和多器官功能障碍(multiple organ dysfunction syndrome,MODS)是创伤、大手术、休克和感染等多种疾病的主要死亡原因之一。本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:问:各位高手,我做细胞培养买了一瓶胰蛋白酶试剂,是amresco的,可上面写的是trypsin 1:250,在金山词霸上查到trypsin是胰岛素的意思,它是否还有胰蛋白酶的意思?多谢! 答:用google(中文搜索)功能,如:通用名 注射用胰蛋白[阅读全文]
摘要:(一)试剂准备1.TRIzol试剂。 2.氯仿 3.异丙醇 4.75%乙醇(DEPC H2O配制) 5.DEPC H2O(二)操作步骤1. 样品处理: (1)培养细胞:收获细胞1-5×107,移入1.5ml离心管中,加入1ml Trizol,混匀,室温静置5min。 (2)组织[阅读全文]
摘要:Q:my group is involved in a national project to develop a genomic platform for cancer research. while Nimblegen has developed target enrichment chip for the Genome Sequencer 20, nothing similar has be[阅读全文]
摘要:This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj[阅读全文]
摘要:The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host [阅读全文]
摘要:IntroductionProlonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor, trichostatin A, specifically affects pericentric heterochromatin. Relocation of thes[阅读全文]
摘要:[阅读全文]
摘要:1.Restrict DNA and gel purify Cut 100ug DNA, removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of Tri[阅读全文]
摘要:DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载:10Tail Prep for Southern Blots.pdf[阅读全文]
摘要:1. Obtain the last 2mm of tail and place directly into 200ul 1X PCR Buffer with Nonionic Detergents (PBND) in a 1.5ml microfuge tube. (Tails can be stored at frozen in PBS or PBND until use.) 2. Add 1[阅读全文]
摘要:NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR. (from Ruixia, 7/99, from protocol by Stef Oehen 7/94). 1. Put 1 cm tail in 1.5 ml microcentrifuge tube. 2. Add 500µl Tail Buffer a[阅读全文]