摘要:ISNT的流式细胞仪分析流程：1．离心收集细胞，PBS洗1～2次　　2．1%多聚甲醛低温下固定15min。　　3．3ml PBS洗1次，70%乙醇固定，冰箱内放置1～3天。　　4．PBS轻洗1次。　　5．2×105细胞与12.5μl的缺口平移缓冲液在15℃共孵育6h，[阅读全文]

摘要:This protocol is based on Shevchenko A,Wilm M,Vorm O,& Mann M.Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels.Anal Chem 1996,68:850-8.I have used it with success on b[阅读全文]

摘要:等离子体发射光谱原理与应用，主要介绍了原子发射光谱发展历史、工作原理等，偏重于仪器方面，应用方面简单做了介绍。《ICP-OES分析原理及应用》下载点击这里下载[阅读全文]

摘要: ICP光谱仪是由ICP光源、进样装置、分光装置、检测器和数据处理系统组成。其中ICP光源由高频发生器、石英炬管和高频感应线圈组成；进样装置是由蠕动泵（一些仪器直接利用同心雾化器提升）、雾化器和雾室等组成；分光装置由入射[阅读全文]

摘要:AAS原理：通过原子化器将待测试样原子化，待测原子吸收待测元素空心阴极灯的光，从而使用检测器检测到的能量变低，从而得到吸光度。吸光度与待测元素的浓度成正比。AAS主要分火焰法和石墨炉法。火焰法现在大家常用的是C2H2+[阅读全文]

摘要:1、【仪器名称】： Image Station多功能成像系统。2、【仪器型号】：IS 2000MM。3、【生产厂家】：Kodak。4、【检测适用范围】：进行活体成像及图像的定量分析处理。5、【仪器使用优点、缺点】：高功率的广谱光源，滤光片选择激发光波长，光纤使[阅读全文]

摘要:IVC指独立通气笼盒。动物实验设施设备是实验动物科学的技术条件保障，而独立通气笼盒（英文缩写IVC）又是一种节能、环保和安全可靠的动物实验设备。1、目的：保持动物有舒适的生活环境，避免动物受病源微生物的影响。2、依据：《[阅读全文]

摘要:实验动物（特别是大型实验动物）的饲养对设备有许多特殊的要求，以确保实验动物的生存条件完全正常，为实验提供正确的结果，另外还要考虑到动物保护组织的意见。EHRET公司的大动物饲养设备正是根据欧美严格的要求，凝聚多年[阅读全文]

摘要:A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell [阅读全文]

摘要:Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week.Soa[阅读全文]

摘要:Hancock Laboratory Methods.Department of Microbiology and Immunology,University of British Columbia,British Columbia,Canadahttp://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=28MATERIALS:Running buffe[阅读全文]

摘要:1)In the cold room,wash cells with cold "Tris" (the same stuff used for TC).Spin down the cells if you are working with suspension cells in a low speed swinging bucket centrifuge,resuspend in approxim[阅读全文]

摘要:1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off PBS.Wash non-adherent cells in PBS and centrifuge at 800 to 1000 rpm in a table-top centrifuge for 5 minutes to pellet [阅读全文]

摘要:Immobilization of ligands on cuvette surfaces and measure the interactions of ligand which is immobilized on the cuvette and the ligate which is added to the immobilized cuvette.Immobilization of liga[阅读全文]

摘要:The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing [阅读全文]

摘要:以抗原免疫动物来制备的抗血清是一个非常复杂的混合物，包括血清的全部成分。但是同抗原特异性结合的抗体则主要是血清中的免疫球蛋白组分。通常用于制备酶标抗体或荧光抗体的免疫球蛋白必须高度纯化并具有特异性，不应含[阅读全文]

摘要:人血浆内的免疫球蛋白大多数存在于丙种球蛋白中。可分为五类，即免疫球蛋白G、免疫球蛋白A、免疫球蛋白M、免疫球蛋白D和免疫球蛋白E。免疫球蛋白是机体受抗原（如病原体）刺激后产生的，其主要作用是与抗原起免疫反应，生成抗[阅读全文]

摘要:Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides, 55°C, 30 min.Xylenes, 2 times, 2 min. each100% EtOH, 2 times, 2 min. each95% EtOH, 2 times, 2 min. each80% EtOH, 2 min.75%[阅读全文]

摘要:1.Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2.Sacrifice the pregnant female mouse (day 13 or 14 p.c.) by cervical dislocation. Dissect out the uterine horn[阅读全文]

摘要:I. General ConsiderationsIn situ hybridization is a powerful technique which can be very informative. It should be however carefully controlled since it can lead to artefacts. One of the most importan[阅读全文]