摘要:PCR产物克隆大致分为两类，即平头连接和粘头连接。平头连接是将制备好的平头载体和补平或削平的PCR 产物直接进行连接。载体可用EcoR V或Sma I切成平头；PCR 产物纯化后，可以在22℃用DNA聚合酶I作用30min（利用该酶所具有的[阅读全文:]
摘要:Oligo designThe 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the PCR f[阅读全文:]
摘要:平端连接通常情况下，PCR产物可直接与平端载体DNA进行连接，但其连接效率效低。因为TaqDNA聚合酶具有非模板依赖性末端转移酶活性，能在两6条DNA链的3'末端加上一个多余的碱基，使合成的PCR产物成为3'突出一个碱基的DNA分[阅读全文:]
摘要:Protocols for HapMap assay-design Affymetrix platform (used by Broad)Defined protocols:LSID:urn:LSID:affymetrix.hapmap.org:Protocol:affy_assay_design_1:1Title: Genotyping using Affymetrix arraysDescri[阅读全文:]
摘要:TA Cloning exploits the terminal transferase activity of some DNA polymerases such as Taq polymerase. This enzyme adds a single, 3'-A overhang to each end of the PCR product. This makes it possible to[阅读全文:]
摘要: The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters a[阅读全文:]
摘要:FootprintingFootprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: hormone-receptor complexes that bind to their hormone response el[阅读全文:]
摘要: 点击浏览该文件[阅读全文:]
摘要:DNaseI FootprintintSolutions10X Binding Buffer200 mM Tris 8.0 200 m l 1M Tris pH 8.0500 mM NaCl 100 m l 5M NaCl10 mM EDTA 20 m l 0.5 M EDTA pH 8.0680 m l Qstore at room temperatureDNaseI Dilution Buff[阅读全文:]