摘要:Preparation of BAC (Bacterial Artificial Chromosome) DNA with CONCERT ™ High Purity Plasmid Purification System1 Courtesy Lisha Xu and Alice C. Young, Molecular and Cell Biology Research and Dev[阅读全文:]
摘要:The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988.Inoculate 2-5 ml of L broth containing the appropriate an[阅读全文:]
摘要:Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand fliesin 0.5 ml lysis buffer (50 mM NaCl , 10 mM EDTA, 50 mM Tris-HCl pH 8). 2.Incubate The sand fly homogenateswith 100 ng/ml R[阅读全文:]
摘要:Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice for isolating double stranded plasmid-based templates for the Sequenase Dye-Labeled Terminator Sequencing Reactions. A. [阅读全文:]
摘要:1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc, pH 7.0 300 ml 1 M NaOAC, pH 7.01 mM EDTA 2 ml 500 mM EDTA, pH 8.0698 ml ddH2O2. Incubate at 22 ℃ for 30 min. Transfer [阅读全文:]
摘要:Important : Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day.1. Add 600 m l of 50 mM [阅读全文:]
摘要:EZ1 病毒迷你试剂盒（ EZ1 Virus Mini Kit ）是一种能够用于生命科学研究中的病毒 DNA 和 RNA 提纯的新型试剂盒。这种试剂盒提供了一种全自动的同步纯化来自血清、血浆和无细胞体液中的病毒 DNA 和 RNA 的能力，并且能对[阅读全文:]
摘要:DESCRIPTIONLabeling of DNA by random oligonucleotide-primed synthesis is based on the investigations of A.Feinberg and B.Vogelstein [1, 2] and is a good alternative to nick translation for producing u[阅读全文:]
摘要:1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at rosette stage if possible). Use 96 well plates (1 or 2 ml, E&K, po[阅读全文:]
摘要:Materials:Silica Suspension: add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2000 x g for 2 minestimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl, 10 mM Tr[阅读全文:]