摘要:Pyrrolizidine alkaloid clivorine inhibits human normal liver L-02 cells growth and activates p38 mitogen-activated protein kinase in L-02 cellsAbstractClivorine, a pyrrolizidine alkaloid extracted fro[阅读全文:]
摘要:摘要：从雄性初断乳SD大鼠肝匀浆中提取肝刺激因子(HSS)并加以部分纯化, 观察其促人肝癌细胞增殖活性及对p21ras蛋白表达的影响。结果表明: (1) HSS具有明显的促人肝癌细胞增殖活性, 其分子量为14～20 kD; (2) HSS可提高p[阅读全文:]
摘要:生命经纬战友suiyi1209 ：BEL-7402 属人肝癌细胞系;以微载体cytodex-3为支持物;使用的培养基为RPMI1640+10%小牛血清;使用倒置相差显微镜,三张图片的放大倍数均为60。倒置相差显微镜显示:图细胞首先贴附到微载体上呈梭[阅读全文:]
摘要:PPARgamma ligands inhibit cholangiocarcinoma cell growth through p53-dependent GADD45 and p21 pathway.Ligands of peroxisome proliferator-activated receptor-gamma (PPARgamma) induce differentiation and[阅读全文:]
摘要:Tumour suppressor p53 and Rb genes in human hepatocellular carcinoma.Aberrations of the p53 and Rb tumour suppressor genes were examined in 12 human hepatocellular carcinoma (HCC)-derived cell lines f[阅读全文:]
摘要:摘要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的：观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法：在细胞培养的不同时间，用α-磷酸奈酚法测定碱性磷酸酶（ALP）活性；放射免[阅读全文:]
摘要:生命经纬网友卡列宁的微笑的观点：用Langendorff灌流消化（胶原酶II）只能取得心肌细胞。建议直接将心脏剪碎，成纤维细胞我们这里一般采用0.05％胰酶消化。无菌条件取出心脏D-hank's液（PBS也可）漂洗，剪碎心脏加入5倍体积消[阅读全文:]
摘要:Neuroendocrine regulation of cyclic AMP formation in osteoblastic cell lines (UMR-106-01, ROS 17/2.8, MC3T3-E1, and Saos-2) and primary bone cells.The effect of four different neuropeptides and norepi[阅读全文:]