摘要:点击下载：20040724151324248.rar[阅读全文:]
摘要:1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca2+/Mg2+ (=400U/ml) and 1 ml into 39 ml HBSS/Ca2+/Mg2+ (=100U/ml). Put on ice. 2. Place 5 ml of the 100U/ml solution in a 10 [阅读全文:]
摘要:Introduction Quality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from [阅读全文:]
摘要:点击下载：cccontamination[1].pdf[阅读全文:]
摘要:Steven Finkbeiner, Departments of Neurology and Physiology, UCSF Protocol note: hood scgedule for day of transfection: [2h] - 6h gap - [1h] CELLS: Polylysine coat plates. add 5ml polylysine per 100mm [阅读全文:]
摘要:Transfect 5 ug of the desired plasmid to the desired adherent cell line. Transfect in parallel GFP and carrier DNA (total of 5 ug). 48hrs after transfection check for transfection efficiency. Taking i[阅读全文:]
摘要:Seed cells at appropriate density (5x104/well for 3T3, MPAC, BHK; 5x105/well for aTC1.6, bTC3) one to two days before transfection. Combine total DNA at 1ug /well with 600ul/well serum-free medium in [阅读全文:]
摘要:Chen-Ming Fan Lab,Carnegie Institute of Washington Prepare cell lysate as you would for Luciferase assay. Take 50uL of lysate and dilute with 100uL of 1X Reporter Lysis buffer (brown bottle at room te[阅读全文:]
摘要:Principle: Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque. During centrifugation, erythr[阅读全文:]