摘要: 图1 革兰氏阳性细菌图2 革兰氏阴性菌（洋葱佰克霍德尔菌）图3 真菌污染图4 真菌污染图5 白色念珠菌（倒置显微镜下）图6 中间长梭型的是正在分裂的念珠菌图7 支原体污染电镜照片(X30k),煎蛋状和其他形状 图8 支原体污染[阅读全文:]
摘要:支原体是一类缺乏细胞壁的原核细胞型微生物，大小一般在0.3-0.5μm之间，呈高度多形性，有球形、杆形、丝状、分枝状等多种形态。它不同于细胞，也不同于病毒。支原体用普通染色法不易着色，用姬姆萨染色很浅，革兰染色为阴性。[阅读全文:]
摘要:MS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0gMOPS..............0.5gAgar..............8.0gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3.[阅读全文:]
摘要:The kitchen makes Tris, TD, Tryp/TD, PBS, and VE. Tris is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and is preferable to TD, unless you need a Ca2+/Mg2+ f[阅读全文:]
摘要:Freezing Cells:1.Trypsinize cells and harvest in the normal way.2.Count a 200 μl aliquot and determine the total cell number.From this,calculate the volume of media required to give a final freezin[阅读全文:]
摘要:METHOD:1.Established hybridomas and other cell lines can be stored in liquid N2 using this method.2.Have everything ready before you start working with the cells (i.e. cool media, label vials, prepare[阅读全文:]
摘要:点击下载：General Guide for Cryogenically Storing.PDF[阅读全文:]
摘要:Procedure1. Pour buffy coat (see Hint #1) from the bag into a 125 ml conical polypropylene centrifuge tube and dilute to 90 ml with sterile endotoxin-free PBS. 2. Add 15 ml of sterile Dextran/PBS and [阅读全文:]
摘要:点击下载：Cryogenic Preservation and Storage of Animal Cells.PDF[阅读全文:]
摘要:MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1 Straight ScissorEtoh Spray bottle1 Bone Scraper1bottle of PBS2 Forceps (1 medium, 1 fine) 5 10 cm plates Sacrifice AnimalsPlace mice[阅读全文:]