摘要:Louis-Bar氏综合征又称共济失调—毛细血管扩张症，为一种特殊类型的原发性免疫缺陷病，是一独立疾病。 【病因和机理】 病因未明。属常染色体隐性遗传。由于DNA修复缺陷，第14对染色体易位所引起，具[阅读全文]

摘要:Lhermitte氏综合征又称前核间型眼肌麻痹综合征、内侧纵束综合征。 【病因和机理】 为内侧纵束在神经膝水平处损害引起。见于多发性硬化及桥脑神经胶质瘤、血管病变、梅毒、酒精中毒与脑干炎症的病人。 【[阅读全文]

摘要:Lennox氏综合征又称Lennox-Gastaut氏综合征、瞬目—点头—跌倒发作、肌阵挛性起立不能小发作等。 【病因和机理】 病因未明。特发性者占10～30%。基础疾病中精神发育迟滞占绝大多数，其次[阅读全文]

摘要:Leigh氏综合征又称Leigh氏脑病、亚急性坏死性脑病、小儿Wernick氏病（误称），是婴幼儿期亚急性地进行性遗传变性疾病。 【病因和机理】 病因未明，可能为常染色体隐性遗传。发现病人脑内三磷酸硫[阅读全文]

摘要:L-门冬酰胺是重要氨基酸，某些肿瘤细胞不能自行合成，需从细胞外摄取。L-门冬酰胺酶（L-asparaginase）可将血清门冬酰胺水解而使肿瘤细胞缺乏门冬酰胺供应，生长受抑。正常细胞能合成门冬酰胺，受[阅读全文]

摘要:Lyme病螺旋体 (Lyme disease spirochete )是疏螺旋体的一种，引起以红斑性丘疹为主的皮肤病变。是以蜱为传播媒介，以野生动物为储存宿主的自然疫源性疾病。该螺旋体是70年代分离出[阅读全文]

摘要:最先从牛肾上腺分离出LDL受体，以后又分离了编码牛LDL受体羟基末端1/3氨基酸的cDNA，并初步阐明了牛LDL受体的cDNA，并且推导出人LDL受体的氨基酸序列。 （一）LDL受体结构 LDL受体是[阅读全文]

摘要:Wear gloves throughout and work in radiation area. Monitor area before and after use.Mix the following in an eppendorf tube:1. 0.5 microgram oligonucleotide dissolved in H2O.2. 3 microliters 10x kinas[阅读全文]

摘要:Linker Ligation (with T4 ) DNA LigaseIn a microcentrifuge tube prepare a solution of blunt ended, dephosphorylated DNA (100-500ng) in TE buffer (5-7µl). Add 1-2µg of phosphorylated linkers[阅读全文]

摘要:Wear gloves throughout and work in radiation area.Monitor area before and after use.Mix the following in an eppendorf tube:1、0.5 microgram oligonucleotide dissolved in H2O.2、3 microliters 10x kinase b[阅读全文]

摘要:Reagents　　10 × tailing buffer (1 M sodium cacodylate, pH 7.0, 10 mM CoCl2, 1 mM DTT)[阅读全文]

摘要: 点击浏览该文件[阅读全文]

摘要:Yale Genome Analysis Center, Yale University http://ygac.med.yale.edu/mtn/LS_transf_prot.stm The following protocols were developed by Petra Ross-Macdonald for Mike Snyder's LacZ fusion project at Yal[阅读全文]

摘要:DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Implementation (by Feng Chen, Hau-Qin Pan and Fu Ying) [阅读全文]

摘要: Hi, Dear All,Have you ever tried to ligate 3 DNA fragments with sticky ends into Vector(5.2kb).A. Fragment: 3.0kb, with HindIII/ Bam HI cut from a Vector.B. Fragment: 0.5kb, BamHI / XbaI cut from a V[阅读全文]

摘要:HiI have a 9kb fragment already cloned into bluescript, and I am trying to insert an extra 3.5kb. I've been trying for some time now with no success - when I do get colonies very few have any of the n[阅读全文]

摘要:David HarryInstitute of Forest GeneticsUSDA Forest Service Pacific Southwest Research StationAugust 26, 1993Background :There are many published methods for mini-preps of DNA from lambda phage cloning[阅读全文]

摘要:Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Karin M,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Bio[阅读全文]

摘要:高效液相色谱作为一种高效、快速的分离分析技术，具有灵敏度高、选择性好的特点，能解决医药分析领域中的诸多复杂问题。如何正确使用及排除故障，将直接影响整个高效液相色谱仪的质量和分析结果的可靠性。本公司使用的LC-10[阅读全文]

摘要:TGEK Base50 mM Tris10% vol Glycerol1 mM EDTA100 mM KCl(add for std lysis buffer):PMSFBenzamidineLeupeptinAprotinin0.5% NP40(add for solubilization buffer)1% NP400.1% Triton X1000.1% SDS0.5% NaDeoxycho[阅读全文]