摘要:Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts and the relative deoxy/dideoxynucleotide ra[阅读全文]

摘要:Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask Phythemaglutinin, PHA-L (Seromed, M 5030) CO2 cell[阅读全文]

摘要: Protocol written by Minoru Toyota 1. Materials 1.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH 8.8 40mM MgCl2 160 mM NH4 (SO4)2 10[阅读全文]

摘要:Hi. I am a new member of this forum. Currently I am trying to look for some novel methylated gene in NPC cell line. What method should I start with? Pls help. Thank you. -CHOO-------------------------[阅读全文]

摘要:Protocol written by James Herman*Methylation-specific PCR (MSP) is a simple rapid and inexpensive method to determine the methylation status of CpG islands. This approach allows the determination of m[阅读全文]

摘要:(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu)A.Large scale double-stranded DNA isolationThe method used for the isola[阅读全文]

摘要:Methylation analyis using restriction enzyme digestionThis method has some weak points.First is that not all CG are located within CCGG sequences,that means many potential methylation sites will be ov[阅读全文]

摘要:Methylated CpG Island AmplificationProtocol written by Minoru Toyota2.Materials2.1.MCARestriction enzymes SmaI,XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl,pH 8.8 40mM[阅读全文]

摘要:Protocol written by James Herman* Methylation-specific PCR (MSP) is a simple rapid and inexpensive method to determine the methylation status of CpG islands. This approach allows the determination of [阅读全文]

摘要:1.While your SDS-PAGE gel is running,make your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikha,top shelf to the right side--looks lik[阅读全文]

摘要:1、开机步骤：1.1打开稳压电源。1.2轻触“ON/OFF键”开启天平。1.3调节底座螺丝，使水平仪中的空气泡位于内圈中部。1.4按“?”键打开玻璃防风罩，按“O/T”键进行去皮并回零。1.5将盛有待测物[阅读全文]

摘要:A. Bst-catalyzed radiolabeled DNA sequencingBst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts[阅读全文]

摘要:Purpose and backgroundsAbout nuclide...35-SDecay: (b-ray,0.167 MeV)Half life: 87.5 daysThick acrylic shield can block most of the emission.Because of the low energy,it is impossible todetect spillage/[阅读全文]

摘要:Hancock Laboratory Methods,Department of Microbiology and Immunology,University of British Columbia, British Columbia, Canadahttp://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=41OBJECTIVE:To methylat[阅读全文]