摘要:Analys of Genomic DNA by Southern Hybridization (Southern Blot) Outline: Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Souther[阅读全文:]
摘要:11、将树脂/DNA 混合液抽干后,加13ml柱子洗脱溶液至离心管中, 对管底部的树脂/DNA 进行洗脱(柱子一边旋转一边加入洗脱液)，并加入柱子中。 12、真空抽干所加入的洗脱。 13、 再加12ml柱子洗脱液进柱子并抽干。 14、 加入5ml [阅读全文:]
摘要:Phenol (removes protein) 1.add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) 2.vortex 3.spin 2 minutes at 12000 rpm 4℃4.transfer supernatant to a fresh tube (avoid aspirati[阅读全文:]
摘要:This protocol is generalized and not to be considered optimal for all strains, conditions, and applications.Some aspects of this protocol may have to be modified significantly to suit your particular [阅读全文:]
摘要:Preparation of Transformation Competent DNA Dilute an overnight bacterial culture 1:20 in L broth. (For E. coli strain JA221, at A )Using a serological pipet, resuspend the bacterial pellet gently in [阅读全文:]
摘要:核酸包括DNA、RNA两种分子，在细胞中都是以与蛋白质结合的状态存在。真核生物的染色体DNA为双链线性分子，原核生物的“染色体”、质粒及真核细胞器DNA为双链环状分子；有些嗜菌体DNA有时为单链环状分子，RNA分子[阅读全文:]
摘要:1、存放：超级感受态细胞必须从干冰运送包装箱取出直接放入–80℃冰箱的底部。一定不要用液氮来存感受态细胞。1) 存放条件：超级感受态细胞对微小的温度改变也极度敏感，因此必须存放在–80℃冰箱的底部。即使是将[阅读全文:]
摘要:碱法大量提取DNA往往需要很长的时间。Promega公司的Wiazrd大量DNA纯化系统既简单又快速，只需要离心和真空抽干，这个系统可以从500ml培养液中在3小时以内获得1mg以上的高质量的质粒DNA(200-20000bp)。该系统不需要酚和氯[阅读全文:]
摘要:Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq. N2 in a mortar. We normally use some alumina to crush hard tissue. Transfer the ground tissue to a eppendorf tube. Add 1 ml [阅读全文:]
摘要:Isolation of DNA from Mouse Tail Biopsies 1. Obtain tail biopsies from 2 to 3 week old mice: Hold mouse firmly at base of tail with one hand, with the other cut off 0.5 to 1.0 cm of the tail tip with [阅读全文:]