摘要:Plate a bacteriophage library at about 30,000-50,000 pfus per 150mm Petri dish by adding an appropriate amount of bacteriophage to 500µl MgSO4-stored E.coli.Dilute bacteria,if needed(ex.VCS237 t[阅读全文:]
摘要:The Erase-a-Base® System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA . The system is based on the procedu[阅读全文:]
摘要:Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest and resuspend cells at 107 / ml in 10 mM Tris-HCl (pH 8.0), 10 mM EDTA. 2.Add SDS and Proteinase K to a final concentrat[阅读全文:]
摘要: Protocol for the cloning of pSHAG-MAGIC2 shRNAsGregory Hannon,Cold spring harbor labpSHAG-MAGIC2.doc [阅读全文:]
摘要:S1 Nuclease Protection Assay End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/mmol)10 µl water + oligo (200 ng)1 µl T4 Kinase1. 37 deg C for 1 h[阅读全文:]
摘要:生物芯片发展至今有很多名称和类型，通常将样品的制备，生化反应、结果的检测和分析这三步不同步骤集成为不同用途的生物芯片，据此分为不同的类型。例如用于样品制备的生物芯片，生化反应生物芯片及各种检测用生物芯片等。[阅读全文:]
摘要:最糟糕的心态-实验失败了，抱怨试剂不好。实验人员本来是应该拥有修正主义、机会主义、怀疑论等诸多“不完美的”意识，所以一定要用“完美的”自我批评意识来平衡。我为什么没有一双慧眼选择可靠的供[阅读全文:]
摘要:For quantitating RNA levels from either polyA+ selected RNA or crude RNA preps.Solutions10X Hybridization Buffer0.4 M PIPES 12.1 g PIPES (Sigma #P6757)4.0 M NaCl 23.4 g NaCl10 mM EDTA 2.0 ml 500 mM ED[阅读全文:]