摘要:质粒具有稳定可靠和操作简便的优点。如果要克隆较小的DNA 片段( ＜10kb) 且结构简单，质粒要比其它任何载体都要好。在质粒载体上进行克隆，从原理上说是很简单的，先用限制性内切酶切割质粒DNA 和目的DNA 片段，然后体外使[阅读全文:]
摘要:1)Prior to any ligation reaction,you should always run one gel in which purified insert and cut backbone are run side by side,preferably beside a known amount of cut DNA.You can then use this to estim[阅读全文:]
摘要: 点击浏览该文件[阅读全文:]
摘要:This procedure is used to analyze inserts in pUC-derived plasimids.1. Suspend E. coli colonies harbouring plasmids in 50 microliters of TE.2. Incubate for 5 min at 95 degrees or in boiling water.3. Mi[阅读全文:]
摘要:1．融化杂合突变ES细胞 ，ES/LIF培养液培养，每2～3天传代，实验开始把细胞 接种到三个100mm铺有凝胶碟皿中，每皿接种1～2×106 细胞 。为筛选需用一个以上的ES杂合突变细胞系，因不同细胞系的转化效率不全相同，另外对检[阅读全文:]
摘要: Immediately before indcution, it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This involves plating of cells on four different plates.Plate[阅读全文:]
摘要:常用的克隆质粒载体，包括：pQE30、pCNDA3、pCDNA3.1、pTYB1、KS等。名称 大小抗性简介PQE303461bpApT5lac启动子，加了6个His可采用chelating sepharose亲和层析纯化。质粒图谱1. http://www1.qiagen.com/ literature/pqesequ[阅读全文:]
摘要:基因工程：有目的的通过分子克隆技术，人为的操作改造基因，改变生物遗传性状的系列过程。载体：能在连接酶的作用下和外源DNA片段连接并运送DNA分子进入受体细胞的DNA分子。转化：指质粒DNA或以它为载体构建的重组DNA导入细[阅读全文:]
摘要:Probes should be stored frozen both when lyopholized or in solution. Aliquoting your probe into additional vials after resuspension can help to minimize potential breakdown due to repeated freezing an[阅读全文:]
摘要:Below we present the protocols we have used to isolate DNA from various tissues using Silica and Guanidinium Thiocyanate.These protocols are adapted from Boom et al. (1990),Höss & Pääbo[阅读全文:]