摘要:Quantitative RT-PCR is an important step for the validation of expression data generated by cDNA microarray screening and other gene discovery techniques. This has been facilitated by the development [阅读全文:]
摘要:METHODS 25, 402–408 (2001)Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2－△△CT MethodKenneth J. Livak* and Thomas D. Schmittgen¶,1*Applied Biosystems, F[阅读全文:]
摘要:DNA/RNA Real-Time Quantitative PCR点击下载：click here .rar[阅读全文:]
摘要:1.Two rounds of DNA synthesis to incorporate fixed sequencesPrepare the following:Reaction tube containing the following:7ml DNA to be amplified and labelled2ml 5X T7 Sequenase Buffer1ml 50mM Pimer 1 [阅读全文:]
摘要:real-time PCR的原理、引物的设计、软件的操作……很实用的一个介绍实时定量PCR的课件。[阅读全文:]
摘要:单核苷酸多态性 ( single nucleotide polymorphisms, SNPs) 主要是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性。SNPs作为第三代遗传标记（已知性、可遗传性、可检测性），用于疾病基因的定位、克隆和鉴定。 本[阅读全文:]
摘要:聚合酶链式反应（Polymerase chain reaction, PCR）实验操作过程有哪些注意事项？还有什么技巧？这个视频，中国制造，简单易懂。[阅读全文:]