摘要:PCR技术又称聚合酶链式反应，最早于1985年由美国Cetus公司人类遗传学研究室Mullis 等人首创，用于扩增DNA。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文:]
摘要:此课件分析了PCR常见问题及解决方法。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文:]
摘要:不能单向强调PCR的优势和检出率应与传统方法比对，合理判断。临床实践是判断PCR结果最重要的依据。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文:]
摘要:这一课件中介绍了PCR在检测结核、HBV等疾病上的应用，并分析了这项技术在临床应用上遇到的问题。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文:]
摘要:Enzymatic reaction relies on fidelity of enzyme to produce a complementary sequence to the template strand in the presence of primers and deoxyribonucleotides.本篇文章来源于网络，如有异议请联系我们，我[阅读全文:]
摘要:分子标记的优点有：数量丰富、信息量大；与生长发育无关，取材不受限制；较多共显性，信息量完整在真核生物中，基因组DNA多态性要远远多于各种基因的遗传多态性，因为基因组中存在着大量的不编码的DNA序列，它们的变异不受或[阅读全文:]
摘要:In this presentation, we will be using Sybr green to monitor DNA synthesis. Sybr green is a dye which binds to double stranded DNA but not to single-stranded DNA and is frequently used to monitor the [阅读全文:]
摘要:The real-time machine not only monitors DNA synthesis during the PCR, it also determines the melting point of the product at the end of the PCR. The melting temperature of a DNA double helix depends o[阅读全文:]
摘要:The polymerase chain reaction (PCR) is invented by K. Mullis in 1985, this technique is used to amplify a sequence of DNA in vitro by simulating the replication procedure of natural DNA in vivo. PCR e[阅读全文:]