摘要:本方法通过SDS裂解细胞，蛋白酶降解蛋白 ，CTAB去除多糖成分。一：仪器：同方法一 二：试剂：TE、TAE缓冲液；10%SDS；5M NaCL；20mg/ml蛋白 酶K；CTAB/NaCL溶液(10% CTAB，0.7M NaCL4.1gNaCL 溶于80ml水中，缓慢加入10gCTAB，加热溶[阅读全文:]
摘要: 限制性内切酶可特异地结合于一段被称为限制酶识别序列的DNA 序列位点上并在此切割双链DNA 。绝大多数限制性内切酶识别长度为4、5或6个核苷酸且呈二重对称的特异序列，切割位点相对于二重对称轴的位置因酶而异。一些酶恰[阅读全文:]
摘要:1 基因芯片技术分离目的基因生物芯片是高密度固定在固相支持介质上的生物信息分子的微列阵。列阵中每个分子的序列及位置都是已知的，并按预先设定好的顺序点阵。基因芯片是生物芯片的一种，其上固定的是核算类物质，主要[阅读全文:]
摘要:DNA WALKING REFERRENCEKi-Bum Park and Suk-Heung Oh(2007)Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK[阅读全文:]
摘要:1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled with LB agar, stabbed with a toothpick which has been put into a bacterial colony). These are then frozen; at -70℃ they wil[阅读全文:]
摘要:The following guidelines should be taken into account when designing modified oligonucleotides. 1.Sequence Length - SYNTHEGEN can synthesize oligonucleotides from 5 to 110 bases in length. Most sequen[阅读全文:]
摘要:FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of:15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digest[阅读全文:]
摘要: An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut o[阅读全文:]
摘要:Protocol:Combine an appropriate concentration of probe that has been labeled on one strand (100-150 fmol probe has worked so far) with the appropriate concentration of protein in a total volume of 50&[阅读全文:]
摘要:Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been utilized by the NCI Prostate Cancer Working Group see the section[阅读全文:]