摘要:This technique can be used to isolate overlapping DNA fragments starting with a previously cloned DNA fragment that maps near a gene of interest (dark red). The walk is continued until a clone contain[阅读全文:]
摘要:Wear gloves throughout and work in radiation area. Monitor area before and after use.Mix the following in an eppendorf tube:1. 0.5 microgram oligonucleotide dissolved in H2O.2. 3 microliters 10x kinas[阅读全文:]
摘要:Protocol for Annealing OligonucleotidesOligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Oligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Annealing Buffer: 10mM Tris, [阅读全文:]
摘要:一、导论 质粒提取的原理:转自复旦大学一位老师的帖子，后面是方法介绍 碱裂解法从大肠杆菌制备质粒，是从事分子生物学研究的实验室每天都要用的常规技术。可是我收研究生十几年了，几乎毫无例外的是我那些给人感觉什么都[阅读全文:]
摘要:1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate at store with 0.02% Sodium azide.2. Plug a 1 mL (blue) pipet tip with Siliconized glass wool. Add 500 ml of 50:50 DE-52 [阅读全文:]
摘要:Salmon Sperm DNA1.Dissolve 1 g salmon sperm DNA in 100 ml H2 O. 2.Autoclave (20 minutes) and aliquot in 1 ml/tube 3.Store at -20℃. (common freezer for stock solutions) [阅读全文:]
摘要:Recovery of DNA from Low Melting Point Agarose Gels1.Run digestion products on 0.7% LMP agarose gel in 1X TBE (it's nice to have at least 1ug of the fragment you want). LMP agarose is fragile; pour ge[阅读全文:]
摘要:酵母 菌基因组 DNA的提取一：仪器： 同方法一二：试剂： SE缓冲液（1M山梨醇，0.1MEDTA pH7.5）；溶菌酶（50mg/ml）；20％PVP；蛋白酶K缓冲液（10mM Tris pH7.60.5% SDS 1mM EDTA）；其余同前三：操作1.5ml对数生长期细菌细胞离心，120[阅读全文:]
摘要:ECKDescriptionThis method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley, 1985). It is a useful way to avoid coprecipitation of proteins and accumulation of salt in the fin[阅读全文:]
摘要:DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus be easily quantitated in a UV spectrometer.Typically, 1 OD260 (i.e. a solution having an absorbance of one unit at 260 n[阅读全文:]