摘要:“展望事业 探讨人生”系列讲座：执着的力量-攻克流感[阅读全文:]
摘要:实验室生物安全管理体系是相互关联或相互作用的一组要素，通过系统性的优化整合而构成的相互协调的有机整体。体系具有关联性、协调性、适应性等特点。体系的真实内涵不易解读，表面上看像是静止、无序的一盘散沙。[阅读全文:]
摘要:一、提取外周血淋巴细胞时注意事项如果取外周血淋巴细胞 (PBMC) 进行ELISPOT检测，最好采用新鲜血液。在保证无菌操作的前提下，首先应保证取血时抗凝剂应及时充分的与血液混匀，避免血凝块的出现。抗凝血最好及时提取PBMC，[阅读全文:]
摘要:Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome and your sample genome. NimbleGen offers two high-d[阅读全文:]
摘要:ALKALINE PHOSPATASE(CIP) DIGESTIONDigestion of protruding 5'-ends1. Precipitate digested DNA and resuspend in a 100ml of 1X CIP Buffer.2. Add 1U CIP for 100pmol 5'-ends.2g linearized 5kb plasmid &Arin[阅读全文:]
摘要:We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hybridization. The genomic DNA was fragmented to 500 to 1000bps before labeling. The following protocol should produce eno[阅读全文:]
摘要:This protocol describes a method for isolating DNA from plant tissue.Procedure1. Preheat the CTAB Isolation Buffer at 60°C.2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a ch[阅读全文:]
摘要:I have been using double-stranded 36mers form STS to probe high-density PAC/BAC filters. The single-stranded overhangs (14 bases) are filled in with 32 P labelled ATP and CTP. POsitive clones are then[阅读全文:]
摘要: Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992)Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8%[阅读全文:]