摘要:DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载：10Tail Prep for Southern Blots.pdf[阅读全文:]
摘要:完整的实验方法请点击下面的链接下载：11 LacZ Cell Staining and β-galactosidase Embryo Staining.pdf [阅读全文:]
摘要:完整的实验方法请点击下面的链接下载：12 Mitotic chromosomes from mouse peripheral blood.pdf[阅读全文:]
摘要:完整的实验方法请点击下面的链接下载：05 Microinjection of fertilized oocytes to produce transgenic animals.pdf[阅读全文:]
摘要:This method works well for 17.5 day mouse embryos. All stains should be made up fresh. Fix embryos in 90% ethanol for at least 1 week, longer if possible. Prior to staining remove skin, and viscera, p[阅读全文:]
摘要:The aggregation method for generating chimaeras, as opposed to the microinjection technique, is useful as it does not require expensive microinjection apparatus or sophisticated manipulative skills, a[阅读全文:]
摘要:SolutionsFixtive using fix buffer to make 0.2% Glutaraldehyde fixative, eg: add 0.2ml 25% Glutaraldehyde (EM grade) into 24.8ml fixing buffer. (fresh make every time) For E10 - E12 embryoDisclaimer: T[阅读全文:]
摘要:Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra[阅读全文:]
摘要:Before you start, have ready the following: - Sterilize 2 pairs of curved forceps, 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES, with an 18[阅读全文:]
摘要:Pre-sterilize two pairs of fine forceps, one pair iris scissors and one blunt-ended 23 gauge needle. Take 3.5 day pregnant mouse, kill and dissect out entire uterus using sterile technique. Place uter[阅读全文:]