摘要:Culture should be 2 days old and colonies should be of medium to large size. This is important as cells need to be in log growth phase. Two small (25cm2) flasks are required, one is harvested in morni[阅读全文:]
摘要:This technique appeared as an article in Biotechniques, Vol.14, No.3, pp412-414. This article is a report by Kristian Gundersen, Theresa Hanley and John Merlie, on the use of micropipette bevelling to[阅读全文:]
摘要:This method is modified from Dr. Lyn Corcoran (WEHI)Disclaimer: The following set of protocols were contributed by various members of our lab (past and present): Christine Andrews, Fiona Christensen, [阅读全文:]
摘要:These protocols have come from the transgenic mouse list and are intended as a guide, none of them have been tried in this lab Cell(s) transferred in small volume of water to a .5 ml tube and lysed by[阅读全文:]
摘要:Injection and Holding PipettesThe glass capillary tubing used should be thin walled, borosilicate glass without a fibre. e.g. Clark Electromedical Instruments Australian Agents: SDR Clinical Technolog[阅读全文:]
摘要:Bleed mice: Obtain 300-400µl from mice of various ages (older mice that have been in the room for an extended period) and strains (include nude mice if in the room). 5-8 mice is a good number. A[阅读全文:]
摘要:Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at [阅读全文:]
摘要:Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for 1-2 electroporations. Procedure 1. Change medium o[阅读全文:]
摘要:Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at [阅读全文:]
摘要:Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 c[阅读全文:]