摘要:When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contamination is bacteria, fungus, mycoplasma, or yeast. [阅读全文:]
摘要:General Annexin V Staining ProcedureSolutions1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a[阅读全文:]
摘要:U251细胞生物学特性Human AstrocytesAstrocytes make up the majority of the cells in the mammalian brain. They are the most variable in type, most intimately associated with all parts of neurons, and thu[阅读全文:]
摘要:树突状细胞(Ｄｅｎｄｒｉｔｉｃｃｅｌｌ,ＤＣ)是机体免疫系统中最强有力的一种专职的抗原呈递细胞(ＡＰＣ),在免疫应答的启动、调控上起着关键的作用[1]。现代抗肿瘤免疫疗法利用ＤＣ负载肿瘤相关抗原(ＴＡＡ)制成的肿瘤疫苗(瘤苗),在转移性前[阅读全文:]
摘要:首先，它肯定不同于原代细胞系的，最大的区别，原代的心肌细胞基本不能分裂，而H9C2可以分裂，最大的区别了吧！原代心肌细胞会跳动，你的H9C2不会吧。虽然都是梭形的，但是H9C2来源于胚胎期心脏，而心肌细胞一般用新生大鼠分离[阅读全文:]
摘要:1.Add 100 l of well-mixed anticoagulated whole blood to the bottom of a labeled tube. 2.Add the appropriate primary antibody to each tube. If using unlabeled antibody, a titration is suggested. Conjug[阅读全文:]
摘要:CHO-K1 (hamster, Chinese, ovary)ECACC 85051005Morphology: EpithelialHamster Chinese ovaryDepositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UKNo restrictions. Patent: None Specified By Deposit[阅读全文:]
摘要:关于293/293T细胞1、种属都为人，不是鼠。2、来源：293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。[阅读全文:]