摘要:MaterialsSuspension cultures set up from Exercise 12.8Sterile transfer pipettesMaterials for viability counting (Exercise 12.7)ProcedureAfter 12 hours, aseptically remove 0.1 ml from each of the three[阅读全文:]
摘要:Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cy[阅读全文:]
摘要:Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted (practical: 200-500 per 0.1 mm3)The chamber of va[阅读全文:]
摘要:Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the back[阅读全文:]
摘要:[摘要] 目的 探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响。方法 用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC-7901、SW-19[阅读全文:]
摘要:1、pcDNA3.1＋-gD的线性化: pcDNA3.1＋使用说明书推荐了以下几个酶作为线性化酶(BglⅡ MfeⅡ Bst1107Ⅰ Eam1105Ⅰ PvuⅠ ScaⅠ SspⅠ),通过DNAMAN分析gD序列发现其带有MfeⅡ酶切位点。2、转染前一天,在60mm的dish中接种8×10[阅读全文:]
摘要:小鼠胚胎成纤维细胞的富集1、给13-14天的孕鼠注射大约0.5ml阿佛丁。当鼠麻醉后，实施断颈法处死小鼠。2、用70％乙醇擦拭腹部，把皮肤向后拉，暴露出腹膜。用消毒过的工具，剪开腹壁以暴露出子宫角。将子宫角移到10cm的皿里。用10[阅读全文:]
摘要:Chemotaxis Assayby Melissa SwopePurpose The purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxi[阅读全文:]
摘要:Mycoplasma Detection Using DNA Staining Mycoplasma_testing_protocol.pdf[阅读全文:]
摘要:General Annexin V Staining ProcedureSolutions1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a[阅读全文:]