摘要:Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final co[阅读全文:]
摘要:After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder of the reaction is concentrated by ethanol precipitation, resuspended in buffer, and subjected to a simultaneous fill-i[阅读全文:]
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摘要:第一节 概 述把一个有用的目的DNA 片段通过重组DNA 技术,送进受体细胞中去进行繁殖和表达的工具叫载体(Vector)。细菌质粒是重组DNA 技术中常用的载体。 质粒(Plasmid)是一种染色体外的稳定遗传因子,大小从1-200kb不等,[阅读全文:]
摘要:This protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data.SolutionsT[阅读全文:]
摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文:]
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摘要:1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl[阅读全文:]
摘要: 1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB with 20µg/ml chloramphenicol and cultured at 37°C with shaking for 16hrs. The liquid bacterial culture is tra[阅读全文:]