摘要: 近10年来，现代分子生物学技术越来越广泛地被用于人类疾病研究的诸领域，为了解病理状态下基因组DNA 的变化积累了新资料。目前认为，人类基因组并非人们想像的那样稳定，诸如基因重排、扩增、缺失，突变和DNA 甲基化类型改[阅读全文:]
摘要:Sample Preparation Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brush[阅读全文:]
摘要:The quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG precipitation procedure (the Core can supplyinformation on this protocol). [阅读全文:]
摘要:1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin for 30 sec .3.decant supernatant and resuspend pellet in 100 ml lysis solution .4.add 200 ml alkaline SDS, vortex well [阅读全文:]
摘要:Day 1 Prepare in advance: Chill plates containing bacteriophage-lysed E. coli for at least 1 hour at 4℃ (2 hours works well).Use 2 nitrocellulose filters for primary screening, and 1 filter after that[阅读全文:]
摘要:Solutions Procedure Design complementary oligonucleotides with compatible half sites on the ends (I use BamHI and BglII). Dry down 300 ng of the two purified oligos together and resuspend in 9 m l Q w[阅读全文:]
摘要:The gel shift, or electrophoretic mobility shift, assay provides a simple and rapid method for detecting DNA -binding proteins. This method has been used widely in the study of sequence-specific DNA -[阅读全文:]
摘要: Materials: g -32 P ATP (New England Nuclear/Perkin Elmer, CAT# NEG-035C)“Upper strand” oligonucleotide (at 0.5 ug/ul)“Lower strand” oliognucleotide (at 0.25 ug/ul)T4 polynucle[阅读全文:]
摘要:For use with GibcoBRL Random Primer DNA labeling system. Objective: To produce radioactively labeled DNA strands for the detection of target DNA or RNA sequences in various applications including Sout[阅读全文:]