摘要:第一节 概 述 把一个有用的目的DNA片段通过重组DNA技术，送进受体细胞中去进行繁殖和表达的工具叫载体(Vector)。细菌质粒是重组DNA技术中常用的载体。 质粒(Plasmid)是一种染色体外的稳定遗传因子，大小从1-200kb不等，为[阅读全文:]
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摘要:This is a modification of a salting out procedure as described by Miller et al., (1988), evaluated at the DNA Laboratory, Medical School, Malta. When analysed by spectrophotometry >95% of extracted ge[阅读全文:]
摘要:Introduction This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocols and[阅读全文:]
摘要:Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high [阅读全文:]
摘要:（一）第一向等电聚焦 1. 从冰箱中取-20℃冷冻保存的水化上样缓冲液（I）（不含DTT，不含Bio-Lyte）一小管（1ml/管），置室温溶解。2. 在小管中加入0.01g DTT， Bio-Lyte 4-6、5-7各2.5ml，充分混匀。3. 从小管中取出400ml水化上样缓[阅读全文:]
摘要:Restriction digests consist of:15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digestions in 96 well plates. Incubate at 37℃ [阅读全文:]
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摘要:Pouring the Gel Outline: Pouring this big & thin 6% acrylamide gel ("Mother of all gels") is quite a challenge and probably the reason why smart whimps by them ready to use.Supplies & Equipment: water[阅读全文:]