摘要:RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types causes degradation of the complementary mRNA (Figure 1[阅读全文:]
摘要:1)Place gene of interest between T7 promoters in Òdouble T7Ó plamsid.(slightly more difficμlt alternative:Place gene of interest in hairpin/inverted repeat configuration behind T7 pro[阅读全文:]
摘要:检测基因特异阻断的最常用的方法是进行westernblot分析，比较导入siRNA 前后蛋白表达水平的变化。在一些情况下可能使用检测报告子基因的报告子系统例如β-半乳糖苷酶。也可以应用实时PCR（例如通过使用LUX™引[阅读全文:]
摘要: 3' R apid A mplification of c DNA E nds (RACE ) PCR This technique is used to obtain the 3'end of a cDNA, it requires some sequence information internal to the mRNA under study. The sequence informat[阅读全文:]
摘要:点击浏览该文件[阅读全文:]
摘要:1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X transcription buffer ( stratagene )1 ml 750 mM DTT1 ml Rnasin (RNase inhibitor; 15U/ml)1 ml 10 mM ATP (pH 7)1 ml 10 mM CTP [阅读全文:]
摘要:Steve Hahn. last modified Sat, Oct 17, 1998Mix the following in an 0.5 ml eppendorf tube:10-20 micrograms total yeast RNA10 microliters hybridization mixH2O to bring the final volume to 25 microliters[阅读全文:]
摘要:Steve HahnLast Modified Fri, Apr 25, 2003Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before use to eliminate [阅读全文:]
摘要:Run-on transcription monitors the regulation of transcription in isolated nuclei.A. Preparation of Nuclei - (do everything at 0℃ to 4℃ in 50 ml tubes)1. Pellet between 30 and 300 million cells at 1,50[阅读全文:]