摘要:In vitro transcription with yeast nuclear extract Steve HahnWear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol befor[阅读全文:]
摘要:一、经乙二醛和二甲基亚砜变性处理后进行的RNA电泳 这一方法原于McMaster和Carmichael(1977)。 含有乙二醛－DMSO的凝胶比含有甲醛的凝胶更难于进行电泳， 因为前者泳动速率较慢而且需将电泳液时行循环以避免电泳过程中形[阅读全文:]
摘要:siRNA Database Searchable database of Silencer ™ Validated and Pre-designed siRNAs to >34,000 human, mouse, and rat targets. All siRNAs in the database have been designed for maximum potency and[阅读全文:]
摘要:MicroRNA and siRNA Cloning Protocol (UPDATED July 2005)[MIT]Bartel Lab, MITthis is a protocol about MicroRNA and siRNA Cloning Protocol collect from internet:http://web.wi.mit.edu/bartel/pub/protocols[阅读全文:]
摘要:Many different methods have been tried in an effort to extract quality RNA from mature conifer needles and phloem, to no avail. Finally here is a method that worked for me. The procedure is adapted fr[阅读全文:]
摘要:High-throughput protein analysis integrating bioinformatics and experimental assays. Nucleic Acids Res. 2004 Feb 3;32(2):742-8 The wealth of transcript information that has been made publicly availabl[阅读全文:]
摘要:人、小鼠和大鼠的多种组织来源的高质量mRNA,经电泳分离后预转于尼龙膜上预制的即用型Northern杂交膜，免去RNA电泳操作过程可检测范围：0.5-10kb可重复使用提供ExpressHybTM快速杂交液样品可靠的优良品质从1991年始，公司[阅读全文:]
摘要:AtlasTM cDNA表达距阵(Expression Array)同时对588种/1176种关键基因做全景表达图谱分析同时对大量已知基因的表达进行大规模高通量(High-htroughput)分析检测关键基因在细胞关键功能中的角色只需简单的杂交步骤就在[阅读全文:]
摘要:Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of the nucleic acid within the gel can be determined by[阅读全文:]