摘要:How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effector step. a | In the first step, input double-strand[阅读全文:]
摘要:RNA干扰(RNAi)是由双链RNA(doublestrandsRNA，dsRNA)引起的，广泛存在于动物植物中的序列特异性转录后基因沉默过程，是生物体在进化过程中，抵御病毒感染及由于重复序列和突变引起基因组不稳定性的保护机制。Elbashir等[1[阅读全文:]
摘要:Design of shRNA inserts for pSHAG-1Gregory Hannon, Cold spring harbor labBseRI-BamHI_Strategy.pdf[阅读全文:]
摘要:We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank> Jack Dixon protocol ( RNAi _Dixon.html" target=_blank>download[阅读全文:]
摘要:The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced[阅读全文:]
摘要:关于RNAi整理贴（基因沉默撰写贴）1.RNAi对照解析贴http://www.biox.cn/bbs/post/view?bid=64&id=7151795&sty=3 http://www.biox.cn/bbs/post/view?bid=64&id=7461187&tpg=1&ppg=1&sty=1&age=0#7461187 http://www.bio[阅读全文:]
摘要:为了获得全长的cDNA 5’及3’末端序列，许多研究人员使用了称之为cDNA末端快速扩增，或RACE的方法。传统的RACE方法得到的PCR产物含有全长及断裂的cDNA产物。GeneRacer™试剂盒确保您只得到含有全长cDNA末[阅读全文:]
摘要: Note:Start with 20 mg of total RNA for each labeling reaction.All solutions that can be filtered should be filtered.Cy dyes are light sensitive and should ALWAYS be handled in dim light.RNA Preparati[阅读全文:]
摘要:3' Rapid Amplification of cDNA Ends (RACE) PCRThis technique is used to obtain the 3'end of a cDNA, it requires some sequence information internal to the mRNA under study. The sequence information obt[阅读全文:]