摘要:电泳法电泳法是指带电荷的供试品（蛋白质、核苷酸等）在惰性支持介质（如纸、醋酸纤维素、琼脂糖凝胶、聚丙烯酰胺凝胶等）中，于电场的作用下，向其对应的电极方向按各自的速度进行泳动，使组分分离成狭窄的区带，用适宜的检测方[阅读全文:]
摘要:1. IntroductionSDS-PAGE (Section 2.2) is probably the most commonly used gel electrophoretic system for analyzing proteins.However, it should be stressed that this method separates denatured protein. [阅读全文:]
摘要:Choose a protein standard MultiMark®SeeBlue® Plus2/SeeBlue®BenchMark™ Pre-stainedMark™BenchMark™MagicMark™ XP newIEF mARKER 3-10ApplicationSDS-PAGEGoodGoodGoodGoodG[阅读全文:]
摘要:Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and interaction with other polypeptides. The migration of proteins depends on many fa[阅读全文:]
摘要: 凝胶干燥时遇到的主要问题是凝胶的变形和破裂。将凝胶放在Whatman 3MM滤纸上可防止干燥凝胶变形，但凝胶是否破裂取决于凝胶的厚度和干燥器的质量，因此，应尽量使用薄胶并使凝胶干燥器处于良好状态，使其真空压力波动极[阅读全文:]
摘要:我们做蛋白质电泳的人都知道，银染色很灵敏，有很强的说服力，但通常胶背景较深，不易扫描。在这里介绍一下低背景的蛋白质银染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min,[阅读全文:]
摘要:NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis.The gels are made up of Bis-Tris-HCl (pH 6.4)polyacrylamide and are intended for denaturing conditions only.NuPAGE Electropho[阅读全文:]
摘要:1.Rinse gel on glass plate briefly with distilled water.2.Wash gel in fresh 50% ETOH-12% HAc,with shaking,3 times,1 hour each.(Fix IEF gel ON in 10% TCA).3.Wash gel four times with 10% ETOH-5% HAc for[阅读全文:]
摘要:SDS-PAGE: gel electrophoresis of proteinsTECHNIQUE is to set up gel plates before you mix the gel mixes.Use the THIN spacers and choose a comb--number of wells varies.Make both resolving gel and stack[阅读全文:]
摘要:Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human,rat and Neurospora (Kull et al.,1996; Sack et al.,1997; Song et al.,2001)and di[阅读全文:]