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摘要:1.Start a 5 ml overnight culture from a frozen stock (in the -70℃)in the following media:For 100 ml of M9CA media tyrp 10 ml of 10X M9 salts5 ml of 10% casamino acids0.2 ml 1M MgSO40.01 ml 1M CaCl20.5[阅读全文]
摘要:This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modificatio[阅读全文]
摘要:毛色白化起源源自远交系Swiss鼠。1935 年,在NIH 饲养,1966 年起开始选育,在接种白日咳疫苗后,其中一系HSFS/N对组胺(Histamine)敏感。70 年代初,在HSFS/N 系第八代发现部份小鼠携带Fv1 b 基因对B strain Friend 白血病[阅读全文]
摘要:Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant material; except for ice water, the solution should be the s[阅读全文]
摘要:F-820血细胞计数仪是日本东亚医用电子株式会社推出的一部用于临床实验室体外诊断的半自动血液分析仪。具有操作简便、软件设计合理、结果较为稳定、准确的特点,适合于门诊检验室使用,现就我们在使用该仪器过程中遇到的一[阅读全文]
摘要:故障现象:一台日本东亚公司的Sysmex F-820全自动血球计数仪,开机时屏幕左下角偶尔出现“HGB AMP ERROR”的故障提示,不能检测HGB项目;如果开机时无此提示,则能正常检测HGB项目;其他检测项目均正常。故障分析[阅读全文]
摘要:使用方法一、打开每个气瓶供应阀到最大;二、把每个流量计定在所需的流量上;“带安全装置的流量计”的操作如下:1.转动O2旋钮。2.当O2,N2O的流量为零时,逆时针转动N2O控制旋钮,增加其流量;O2控制旋钮也按预定转[阅读全文]
摘要:目的:制定FA1104型电子天平的使用标准操作规程,确保操作的规范性、正确性,结果的可靠性。1、使用前准备1.1 使用之前,要预先向管理人员做好预约,做好使用登记。1.2 称重工作台前一定要保持平稳牢固可靠。1.3 用水仪调整调[阅读全文]
摘要:操作过程1.把轮鼓边螺丝拧开,放入样品再拧上螺丝。2.打开仪器电源开关,按一下“COUNTER/TIMER(计时/计数)”键选择计时或计数。3.按“SET(置位)”键,显示屏从最后一位开始闪烁,每按一次“SET([阅读全文]
摘要:What it Introduction “Flow” + “Cell” + “Measurement” = FCM (FACS®) does: Measures cell functionalities and quantities (Fluorescence) Measures cell sizes (sc[阅读全文]
摘要:Specimen collection:Whole blood is collected in Vacutainer CPT (Becton-Dickinson) or 9 ml EDTA vials.Isolation of peripheral mononuclear cells:If CPT Vacutainer are used, we follow in principle the VA[阅读全文]
摘要:文件简介: 点击下面的链接查看全文:fitting brendan five parameter logistic curve.PDF[阅读全文]
摘要:流式细胞仪是进行流式细胞分析的仪器,集电子、计算机、激光、流体理论于一体。流式细胞术(Flow CytoMeter, FCM)是一种在功能水平上对单细胞或其他生物粒子进行定量分析和分选的检测手段,它可高速分析上万个细胞,并能同时[阅读全文]
摘要:Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells by adding 4 ml of -20°C absolute ethanol.[阅读全文]
摘要:BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-bindin[阅读全文]
摘要:Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cy[阅读全文]
摘要:Freezing Cells:1.Trypsinize cells and harvest in the normal way.2.Count a 200 μl aliquot and determine the total cell number.From this,calculate the volume of media required to give a final freezin[阅读全文]
摘要:FACSCalibur的使用程序点击下面的链接下载:FACSCalibur 的使用程序.pdf[阅读全文]