摘要:Bleed mice: Obtain 300-400µl from mice of various ages (older mice that have been in the room for an extended period) and strains (include nude mice if in the room). 5-8 mice is a good number. A[阅读全文]

摘要:PCR screens must be designed to detect transgene DNA at the single copy level. Southern Blots analysis of transgenic mice need copy standards to estimate copy number. Copy standards are prepared by mi[阅读全文]

摘要:Reagents Avidin-Cy5 (1.8 mg/ml)Bovine Serum Albumin (BSA)DAPIFormamideHCl, 1 NMouse anti-digoxigenin (0.1 mg/ml)Sheep anti-mouse Cy5.5 (1.0mg/ml)SSC, 20XTween 20dH2O 详细操作流程请点击下面的链接下载：S[阅读全文]

摘要: DNA的提取与检测人和哺乳动物细胞基因组DNA的分离通常是在有EDTA、Sarkosye等一类去污剂存在下，用蛋白酶K消化细胞，随后用酚、氯仿抽提，经RNase处理和纯化来提[阅读全文]