摘要:1、在长波紫外灯下切下含有目标DNA的琼脂糖凝胶， 用纸巾吸干凝胶表面的液体并切小。计算凝胶的重量。2、加入2-3倍体积的6M KI或NaI（避光4度保存）。3、温浴， 间断混合，直到凝胶熔化。4、加适量的振匀的silica悬液（10ul）充分混[阅读全文]

摘要:两个人的基因只有0.1%是不同的，大多数多样性采取SNPs的形式。[阅读全文]

摘要:一、简介：SSR简单序列重复标记（Simple sequence repeat, 简称SSR标记），也叫微卫星序列重复，是由一类由几个核苷酸（1-5个）为重复单位组成的长达几十个核苷酸的重复序列，长度较短，广泛分布在染色体上。由于重复单位的次数[阅读全文]

摘要:Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed the silencing efficiency of siRNA duplexes as a fun[阅读全文]

摘要:In the past, poly(A)+ RNA has not been detected in prokaryotes.In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and pot[阅读全文]

摘要:Secondary structure of prion mRNALuck R; Steger G; Riesner D Institut fur Physikalische Biologie, Heinrich-Heine-Universitat, Dusseldorf, FRG.J Mol Biol 258: 813-26 (1996) An algorithm for prediction [阅读全文]

摘要:The siRNA user guide(revised May 6, 2004)Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed the silenc[阅读全文]

摘要:Our current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western blotting. This allows us to test several candidate siRNAs quite c[阅读全文]

摘要:RNAi target selection rules : Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG). Search for sequence motif AA(N19 )TT or NA(N21 )[阅读全文]

摘要:越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链RNA分子，能够以同源互补序列的mRNA为靶目标降解特定的mRNA，这个过程就是RNA干扰途径（R[阅读全文]

摘要:Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthesis, in vitro transcription, siRNA expression vect[阅读全文]

摘要:siRNA Design RNAi target selection rules :Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG). Search for sequence motif AA(N19 )TT[阅读全文]

摘要: A．普通阴性对照1.siRNA实验应该有阴性对照；2.通用阴性对照为与目的基因的序列无同源性的普通阴性对照；3．Scrambled阴性对照和选中的siRNA序列有相同的组成，但是和mRNA没有明显的同源性；4．阴性对照需要确定和目的靶细[阅读全文]

摘要:将制备好的siRNA，siRNA表达载体或表达框架转导至真核细胞 中的方法主要有以下几种： 1.磷酸钙共沉淀将氯化钙，RNA(或DNA )和磷酸缓冲液混合，沉淀形成包含DNA 且极小的不溶的磷酸钙颗粒。磷酸钙-DNA 复合物粘附到细胞 膜[阅读全文]

摘要:1．希望软件可以在 web 上使用。 即用户可以访问我们的网站， 输入基因代码， 即可自行在网上设计 siRNA Oligo 。2．可参照的软件形式： 见如下网站： www.dharmacon.comwww.ambion.comwww.qiagen.com3．希望能就每一个所指定[阅读全文]

摘要:1.NCBIshRNA资源库 http://cgap.nci.nih.gov/RNAi About RNAi on CGAPThe NCBI is part of the consortium supporting the preparation of human and mouse libraries containing RNAi constructs that target cance[阅读全文]

摘要:Steve Hahn. last modified Sat, Oct 17, 1998Mix the following in an 0.5 ml eppendorf tube:10-20 micrograms total yeast RNA10 microliters hybridization mixH2O to bring the final volume to 25 microliters[阅读全文]

摘要:siRNA Design RNAi target selection rules :Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG). Search for sequence motif AA(N19 )TT[阅读全文]

摘要:Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating the results by real-time RT-PCR. Ambion's Silencer&[阅读全文]

摘要:在活体RNAi关键点：类似药物属性的引入，如稳定性、细胞内的传送、组织的生物可溶性进入合成的siRNA。授予siRNA类似药物的属性化学稳定性的胆固醇共聚的siRNA 显著的改善了其在体内和体外的药物属性。化学稳定性增强的方法[阅读全文]