摘要:一、 概述电热恒温培养箱、隔水式培养箱是供医疗卫生、医药、农业、科研等部门作储藏菌种、生物培养，是科学研究的必须设备。二、 产品特点1、 箱体外壳由优质钢板制成，外表喷塑美观大方，工作室采用不锈钢制成。2、 门中有玻璃观[阅读全文]

摘要:SPA提取的方法很多，包括煮沸法、溶菌酶法、葡萄球菌溶素法、超生波法以及三氯醋酸法等。现将常用的方法介绍如下：1.煮沸提取法⑴ 将菌株接种于蛋白胨葡萄糖琼脂培养基上，37℃培养20h~24h。　　⑵ 以0.15mol/L pH5.9PB液将菌苔洗[阅读全文]

摘要:一、实验原理与意义免疫组织化学又称免疫细胞化学，是指带显色剂标记的特异性抗体在组织细胞原位通过抗原抗体反应和组织化学的呈色反应，对相应抗原进行定性、定位、定量测定的一项新技术。SP(streptavidin-perosidase)法，[阅读全文]

摘要:金黄色葡萄球菌A蛋白（staphylococcal protein A，SPA）可与IC中IgG的Fc段结合。将待测血清用低浓度PEG沉淀后加至SPA包被的固相载体上，再以酶标记的SPA与之反应，即可检测样本中有无IC。1、试剂（1）5%与2.5%PEG用0.02mol/LpH[阅读全文]

摘要:内容提要用免疫印迹法检测134例SLE患者多肽抗体谱，阳性率71.7%，其中Sm抗体37.3%，RNP抗体21.6%，SS-A抗体24.6%，SS-B抗体20.1%，核糖体20.1%。本法对SLE诊断、分型有一定临床价值。系统性红斑狼疮（SLE）的血清学特征是出现多[阅读全文]

摘要:A．Materials1. NUNC-Immuno Plate IIF. 2. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) 3. PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin. 4. BSA (1% in P[阅读全文]

摘要:It is possible to combine variations in density and size to allow for separation of cell populations within the earth's gravitational field, i.e. without a centrifuge. A technique devised by Miller an[阅读全文]

摘要:Materials Table2 Density and refractive indexes of sucroseTable1 Densities of cell structures in sucrose Sucrose density fractions from Exercise“Sucrose Fractionation of Castor Bean”Proced[阅读全文]

摘要:Materials Castor beansGradient former Sucrose solutions in 0.01 M EDTA, pH 7.533%, 44%, 50%, 57%, 60% (w/v)Grinding Medium0.4 M sucrose0.165 M Tris-HCl buffer, pH 7.50.01 M KCl0.01 M MgCl0.01 M EDTA a[阅读全文]

摘要:1.Basic Techniques - The 'Do's and Don'ts' of Cell CultureGiven below are a few of the essential "do's and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipmen[阅读全文]

摘要:How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum at -70º C, as it does not prolong [阅读全文]

摘要:Whole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluores[阅读全文]

摘要:BrdU Incorporation ProtocolDay 1Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours ES cells, 30 minutes 3T3 cells, 2.5 hours Trypsinize cells[阅读全文]

摘要:1.白细胞五分类 2.流式细胞检测 3.无须前处理即可进行全自动网织红细胞测定。具备网织红细胞专用仪器所具备的特殊处理能力，实现高效，高精度分析。 [阅读全文]

摘要:The kitchen makes Tris, TD, Tryp/TD, PBS, and VE. Tris is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and is preferable to TD, unless you need a Ca2+/Mg2+ f[阅读全文]

摘要:1.Risk AssessmentThe main aim of risk assessment is to prevent injury, protect property and avoid harm to individuals and the environment. The performance of risk assessment is a legal requirement und[阅读全文]

摘要:Large numbers of cell lines look identical. Cell lines with very different origins and biological characteristics typically cannot be separated on grounds of morphology or culture characteristics. Inf[阅读全文]

摘要:请问SiRNA 能否与质粒DNA一起转染细胞？应该注意什么、如何进行无酶处理？一般用什么转染试剂？我手头的SiRNA和转染试剂是dharmacon的。答：我们这边siRNA和质粒DNA共转染用lipo2000。请问怎样防止RNA降解，RNA与质粒的比率[阅读全文]

摘要:SP 2/0是小鼠骨髓瘤细胞株，贴壁生长，科室给学生开实验就用此细胞，所用培养基为1640，用小牛血清，浓度15%生长较好。培养孵箱为37度，5%的CO2。开始将复苏的细胞传到小培养瓶（如25ml)，由于细胞分泌各种因子及细胞间的相互[阅读全文]

摘要:自CO2培养箱取出培养瓶。超净工作台中 以吸管轻轻吹打有细胞的瓶壁，去除生长不好的细胞。弃去培养液。加0.04% EDTA数滴 (用0.02%的EDTA不易消化掉) ，浸过细胞。置37℃培养箱10min 左右。取出，倒置显微镜下观察，细胞变圆回[阅读全文]