摘要:293细胞是腺病毒载体的包装细胞。腺病毒是继逆转录病毒后用于基因治疗研究的热门载体。直接将腺病毒载体导入人体内表达目的基因，治疗恶性肿瘤，心血管疾病或一些遗传疾病已取得可喜进展；利用腺病毒载体在包装细胞 293中[阅读全文:]
摘要:I. General ConsiderationsIn situ hybridization is a powerful technique which can be very informative. It should be however carefully controlled since it can lead to artefacts. One of the most importan[阅读全文:]
摘要:1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml .2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 [阅读全文:]
摘要:Phosphate-buffered saline (PBS) (0.01 M Na2HPO4 , 0.15 M NaCl, pH 7.4):sodium phosphate dibasic (Na2HPO4) 0.87 gsodium phosphate monobasic (NaH2PO4 x H2O) 0.54 gsodium chloride 8.5 gHPLC water 1 liter[阅读全文:]
摘要:Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, they should be centrifuged at a 64.4 xg on the IEC cl[阅读全文:]
摘要:Laser Scanning Cytometry激光扫描细胞仪（影像细胞仪）拥有流式细胞仪的数据分析能力，但检测的样本是放置在载玻片或96孔板或其他各类型透明介质。它可以像流式细胞仪一样定量检测荧光信号并能够观察细胞形态。苏州大学[阅读全文:]
摘要:自身免疫性疾病主要特征是免疫系统在识别“自我”与“非我”时出现差错。尽管绝大多数自身免疫性T细胞在胸腺成熟过程中被调节分化后去除，但正常人体中总是带有部分自身反应性细胞。调节性T细胞是[阅读全文:]
摘要:肿瘤细胞在组织培养中占有核心的位置，首先癌细胞是比较容易培养的细胞。当前建立的细胞系中癌细胞系是最多的。另外肿瘤对人类是威胁最大的疾病。肿瘤细胞培养是研究癌变机理、抗癌药检测、癌分子生物学极其重要的手段。肿[阅读全文:]