摘要:PCR:We generally use 1-2 ul of starting paraffin microdissected DNA for each 50 ul DOP-PCR reaction.We assume that about 1 ug of product is produced in each DOP-PCR reaction. The entire product is the[阅读全文:]
摘要:This procedure will work for both yeast and E. coli:Take a small colony and suspend it in 5ul of H2O in a PCR tube. Heat for 5 min at 95℃ and then spin the condensation down in a microfuge. Set up the[阅读全文:]
摘要:1,Basic PrinciplesThe requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperat[阅读全文:]
摘要:A method to re-PCR unique bands from products of mixed sizeINTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - ofte[阅读全文:]
摘要:In this experiment, polymerase chain reaction (PCR) is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen[阅读全文:]
摘要:1、Marker选择标准（1）应选择在目标片段大小附近ladder较密的marker，这样对目标片段大小的估计较准确。（2）所选marker应能清楚反映目标片段的大小，且次要片段大小也能反映出来。如作酶切鉴定时，目的片段和切后载体片段最[阅读全文:]
摘要:聚合酶链反应(Polymerase Chain Reaction ,PCR)是80年代中期发展起来的体外核酸扩增技术。它具有特异、敏感、产率高、快速、简便、重复性好、易自动化等突出优点；能在一个试管内将所要研究的目的基因或某一DNA片段于数小时[阅读全文:]
摘要:1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of interest with primers about 200 bases apart. Primer 2 should begin with 24 nts complementary to the M13 forward primer(GTC GTG A[阅读全文:]
摘要:1,IntroductionMore than 15 years after its initial description, the polymerase chain reaction (PCR) has become a standard molecular biology tool and is the most widely used method of nucleic acid ampl[阅读全文:]
摘要:由Mullis等在1983年建立的PCR技术已成为分子生物学、遗传学等学科研究领域的经典实验方法。近年来,该技术本身获得了长足发展,可靠性不断提高,在聚合酶链式反应基本原理的基础上,发展了一系列新的概念和实验方法,在生命[阅读全文:]