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搜索“TAG:s”找到相关内容264篇,用时0.020374秒
摘要:DNA Transfer1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7' (large gels)2. Denaturation: Wash gel in (0.5 M NaOH, 1.5 M NaCl) for 20' (small) to 30' (large).3. Neutralizat[阅读全文]
摘要:1.Digest DNA: • 1.5 μg DNA in a 25 μI reaction containing BSA• 3 to 5 units for 5 to 3 hours (aim for 10-fold overdigestion; remember many enzymes will not survive well for more than 3[阅读全文]
摘要:【原理】Southern 印迹是将电泳分离的 DNA 片段转移到一定的固相支持物上的过程。DNA 分子经限制性核酸内切酶酶切,经琼脂糖凝胶电泳将得 DNA 片段按分子量大小分离,然后将含 DNA 片段的琼脂糖凝胶变性,并将单链 DNA 片[阅读全文]
摘要:DNA PrepPrepare DNA via your favorite method.You may find a protocol underMini Yeast Genomic Prep.Restriction Digest1.Digest DNA with appropriate restriction enzyme.Digest 10μg of Yeastchromosomal [阅读全文]
摘要:Hi,Can you tell me what do "SSC" in a southern blot, how he act on DNA or in the protocole.(excuse-me for my traduction, i never write in english)-Sebela-"SSC" is a abbreviation of sodium chloride and[阅读全文]
摘要:1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent to the molecular weight markers as a reference.3. [阅读全文]
摘要:The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoF[阅读全文]
摘要: There are several ways to prepare DNA from plants. I have found this method to be extremely efficient and quick. The DNA prepared via this protocol can be used in any PCR reaction. However, the DNA f[阅读全文]
摘要:Southern Analysis Procedure (for analysis of genomic DNA or ordering of clones): 1) Prepare genomic DNA (ref.p.9.22, Maniatis) from cultured cells using one T75 flask (wash two times with PBS and then[阅读全文]
摘要:Silica Suspension: add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2000 x g for 2 minestimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 [阅读全文]
摘要:sanger是英国生物化学家,1918年8月13日生于英格兰格洛斯特夏郡,在剑桥大学圣· 约翰学院获哲学博士学位,毕业后到著名的剑桥医学研究会分子生物学实验室工作。Sanger的工作主要研究蛋白质的结构,特别是研究测定[阅读全文]
摘要:用碱和SDS处理可以从中度规模(20~50ml)的细菌培养物中分离质粒DNA,所获得的DNA产物可用于电泳分析或限制性内切核酸酶消化,经柱层析进一步纯化之后,还可用于转染哺乳动物细胞。 [阅读全文]
摘要:碱和SDS处理可以从中度规模(20~50ml)的细菌培养物中分离质粒DNA,所获得的DNA产物可用于电泳分析或限制性内切核酸消化,经柱层析进一步纯化之后,还可用于转染哺乳动物细胞。[阅读全文]
摘要:这种温和的方法在处理大质粒(>15kb)时较碱裂解法和煮沸法好。但温和的代价是产量低:有相当数量的质粒DNA混于细胞渣滓中,在操作的早期步骤中丢失。 [阅读全文]
摘要:核酸分子杂交技术用一段核酸片段带上特殊标记物后,在一定温度,离子强度,PH条件下,与相同or相似的核酸分子以硷基互补原则(C≡G, A=T,A=U)形成新的杂合分子的过程,以检测核酸分子的存在。根据反应性质分为:RNA-RNA, RNA-[阅读全文]
摘要:介绍SNP(单核苷酸多态性)的动画和意义SNP(单核苷酸多态性)[阅读全文]
摘要:1、取0.1~0.2 g左右小麦叶片于1.5 ml的Eppendorf离心管中,将离心管直接放入盛液氮的冰壶中冷冻。从冰壶中取出离心管,用小玻璃研棒直接插入离心管中迅速研磨,至淡黄色粉末为宜;2、向离心管中加入600 ml的DNA提取缓冲液S,[阅读全文]