摘要:Assemble the following components: •Cells,grown in 10 cm dishes •Room Temp.PBS •Cold PBS •Cold Sonication Buffer (1% SDS,10 mM EDTA,50 mM Tris-HCl pH 7.5)Prepare sonication buffer [阅读全文:]
摘要:Upstate公司在世界上最先开发出步骤优化简洁的商业化ChIP分析试剂盒，保证实验的准确性和可重复性，同时Upstate提供广泛可行的ChIP级别应用抗体。ChIP基本试剂盒和特定蛋白 质ChIP级别应用抗体结合完成一个完整的染色质[阅读全文:]
摘要:This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj[阅读全文:]
摘要:采用OG05 差异显示技术，分析水稻稻瘟病抗源材料“ 地谷”叶片受稻瘟病菌侵染前后的基因的表达差异，获得87个差异片段。对这87个差异片段进行了回收、重扩增与克隆，并对其中的81个片段进行了杂交鉴定。斑点杂交[阅读全文:]
摘要:All living organisms have thousands to tens of thousands of unique genes encoded in their genome, of which only a small fraction, perhaps 15%, are expressed in any individual cell. Therefore, it is th[阅读全文:]
摘要:抑制性消减杂交(suppression subtractive hybridization， SSH)技术是一种鉴定、分离组织细胞中选择性表达基因的技术. 其原理是以抑制性多聚酶链反应(PCR)反应为基础的cDNA 消减杂交技术. 通过合成两个不同的接头，连接[阅读全文:]
摘要:1、一般培养——保持胚胎干细胞处于未分化状态培养基细胞复苏冻存细胞明胶包被细胞传代2 、体外分化培养基：包被有多聚鸟氨酸/纤维结合蛋白的培养板（使用或不使用盖玻片）体外分化方法注：以下培养针对于小鼠的[阅读全文:]
摘要:组织原位杂交已广泛应用于植物组织、细胞的基因时空表达研究。目前主要有3种方法：一是常规组织切片原位杂交，广泛适用于各种组织；二是大量细胞的整体原位杂交，已应用于花粉管和藻类合子；三是微量细胞的整体原位杂交，用[阅读全文:]
摘要:点击查看PDF文件[阅读全文:]
摘要:The number of infertile couples has increased in recent years and is now estimated at 15–20% of all couples worldwide. Since its introduction into reproductive medicine in 1978 [1], in vitro fer[阅读全文:]