摘要:SolutionsCPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl2 up to 100 ml with Q store at room temperature 1M KPO4 pH 7.5 250 ml 1M KH2PO4 (monobasic) 68.05 g up to 500 [阅读全文:]
摘要:The number of infertile couples has increased in recent years and is now estimated at 15–20% of all couples worldwide. Since its introduction into reproductive medicine in 1978 [1], in vitro fer[阅读全文:]
摘要:2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control.Replace 1X PBS solution over biopsy with 2.[阅读全文:]
摘要:General Comments Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm[阅读全文:]
摘要:The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host [阅读全文:]
摘要:文件预览:点击下面的链接下载完整protocol:knockout.doc[阅读全文:]
摘要:1-cell embryo transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - those that have been damaged during t[阅读全文:]
摘要:Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (28,4g/l[阅读全文:]
摘要:Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Australia [阅读全文:]
摘要:Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra[阅读全文:]