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Intracytoplasmic Spermatocyte Injection (ICSI)(图)
词条创建者:admin创建时间:12-06 23:14
标签: Intracytoplasmic Spermatocyte Injection ICSI

摘要:The number of infertile couples has increased in recent years and is now estimated at 15–20% of all couples worldwide. Since its introduction into reproductive medicine in 1978 [1], in vitro fer[阅读全文:]

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ES injection pipette manufacture(图)
词条创建者:admin创建时间:12-06 23:14
标签: ES injection pipette

摘要:The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor[阅读全文:]

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Lac-Z Detection in Tail Biopsies
词条创建者:admin创建时间:12-06 23:14
标签: Lac-Z Detection

摘要:2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control.Replace 1X PBS solution over biopsy with 2.[阅读全文:]

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Sample Preparation for Microinjection
词条创建者:admin创建时间:12-06 23:14
标签: Sample Preparation Microinjection

摘要:General Comments Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm[阅读全文:]

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Transgenic聽Animals
词条创建者:admin创建时间:12-06 23:14
标签: Transgenic Animals

摘要:The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host [阅读全文:]

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1-cell embryo transfer into pseudopregnant recipient female mouse(图)
词条创建者:admin创建时间:12-06 23:14
标签: 1-cell embryo transfer pseudopregnant recipient mouse

摘要:1-cell embryo transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - those that have been damaged during t[阅读全文:]

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C. elegans Gene Knockout Protocol
词条创建者:admin创建时间:12-06 23:14
标签: C. elegans Gene Knockout

摘要:文件预览:点击下面的链接下载完整protocol:knockout.doc[阅读全文:]

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b-Gal Staining of Murine Embryos
词条创建者:admin创建时间:12-06 23:14
标签: b-Gal Staining Murine Embryos

摘要:Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (28,4g/l[阅读全文:]

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Embryo Manipulation Pipettes (Flushers etc.)
词条创建者:admin创建时间:12-06 23:14
标签: Embryo Manipulation Pipettes

摘要:Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Australia [阅读全文:]

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Blastocyst Transfer
词条创建者:admin创建时间:12-06 23:14
标签: Blastocyst Transfer

摘要:Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra[阅读全文:]

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遗传学技术