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Purification of Gene Targeting Vector DNA for Electroporation
词条创建者:admin创建时间:12-06 23:14
标签: Transgene Mice DNA Preparation

摘要:1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electrop[阅读全文:]

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Microinjection DNA Purification
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice DNA Preparation

摘要:We have found the UltraClean™ GelSpin™ Kit (available from Mo Bio Laboratories, Catalog number 12400-250) is a simple and fast way to obtain microinjection quality DNA. This is not to excl[阅读全文:]

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Transgenic Mouse Core Facility
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice DNA Preparation

摘要:1.Restrict DNA and gel purify Cut 100ug DNA, removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of Tri[阅读全文:]

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DNA ultrapurification
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice DNA Preparation

摘要:The success of DNA purification can have profound consequences on the success or failure of any injection effort. This summary of techniques from several different sources was compiled by Brad Preston[阅读全文:]

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Preparation of Transgene DNA for Microinjection
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice DNA Preparation

摘要:The purity of the transgene DNA used for microinjection is critical for the successful production of transgenic founder mice. DNA impurities in the form of bacterial endotoxins or organic contaminants[阅读全文:]

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AP Assay and FGF Binding
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification

摘要:FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of pro[阅读全文:]

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Combined LacZ staining and in situ hybridization
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification

摘要:Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal. 完[阅读全文:]

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G-banding for analysing mouse chromosomal rearrangements
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification

摘要:完整的实验方法请点击下面的链接下载:09G-banding for analysing mouse chromosomal rearrangements.pdf[阅读全文:]

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Embedding, sectioning and counterstaining of LacZ embryos
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification

摘要:完整的实验方法请点击下面的链接下载:08Embedding, sectioning and counterstaining of LacZ embryos.pdf[阅读全文:]

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Tail Prep for Southern Blots
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification Southern Blots

摘要:DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载:10Tail Prep for Southern Blots.pdf[阅读全文:]

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遗传学技术