摘要:MaterialsPrepared slides of Ascaris megalocephala maturationMicroscopeProcedure1.Place the slide(s) of Ascaris on the microscope and focus on the eggs within the body.Unlike the grasshopper testis whe[阅读全文:]
摘要:1、 糖发酵管成分: 牛肉膏 5g 蛋白胨 10g 氯化钠 3g 磷酸氢二钠(Na2HPO4•12H2O) 2g 0.2%滇麝香草酚蓝溶液 12ml 蒸馏水 1000ml pH7.4制法: 1、葡萄糖发酵管按上述成分配好后,按0.5%加入葡萄糖,分装于有一[阅读全文:]
摘要:MaterialsMonolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used, or cultures of tetrahymena, yeast, or algae may be used.)H-thymidine with at least 4 µc/ml, 0.36 [阅读全文:]
摘要:MaterialsGrowing root tips of the broad bean,V. faba (2n=12)Solutions of chromosome-damaging agents listed in the introduction to this ChapterReagents for Feulgen stain (Exercise 2.5)Oven at 60° C[阅读全文:]
摘要:11、苯丙氨酸培养基成分: 酵母浸膏 3g DI-苯丙氨酸(或L-苯丙氨酸1g) 2g 磷酸氢二钠 1g 氯化钠 5g 琼脂 12g 蒸馏水 1000ml制法:加热溶解后分装试管,121℃高压灭菌15min,使成斜面。试验方法:自琼脂斜面上挑取大量培[阅读全文:]
摘要:31营养琼脂成分:蛋白胨 10g牛肉膏 3g 氯化钠 5g琼脂 15~20g蒸馏水 1000mL制法:将除琼脂以外的各成分溶解于蒸馏水内,加入15%氢氧化钠溶液约2mL,校正pH至7.2~7.4。加入琼脂,加热煮沸,使琼脂溶化。分装烧瓶,121℃高压灭菌1[阅读全文:]
摘要:21 过氧化物酶试验试剂:2%儿茶酚溶液。3%过氧化氢溶液。试验方法:挑取固体培养基上菌落一接种环,置于洁净试管内,滴加2%儿茶酚溶液1mL及3%过氧化氢溶液1mL。静置于室温(20℃)中30~60min,观察结果。结果:阳性反应,细菌变为[阅读全文:]
摘要:51 三糖铁琼脂(换用方法)成分:蛋白胨 15g遍胨 5g牛肉膏 3g酵母膏 3g乳糖 10g蔗糖 10g葡萄糖 1g氯化钠 5g硫酸亚铁 0.2g硫代硫酸钠 0.3g琼脂 12g酚红 0.025g蒸馏水 1000mLpH7.4制法:将除琼脂和酚红以外的各成分溶解于[阅读全文:]
摘要:62 氯化钠结晶紫增菌液成分:蛋白胨 20g氯化钠 40g0.01%结晶紫溶液 5mL蒸馏水 1000mLpH9.0制法:除结晶紫外,其他按上述成分:配好,加热溶解。约加30%氢氧化钾溶液4。5mL,校正pH。加热煮沸,过滤。再加入结晶紫溶液,混合后分[阅读全文:]
摘要:IntroductionA major advance in our knowledge of cells came about with the ability to maintain them in continous culture. Prokaryotes have been cultured for a relatively long time, but eukaryotic cultu[阅读全文:]